A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy

Aishwarya Sathyanarayan

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Aberrant liver function as a consequence of excessive fat accumulation as observed in non-alcoholic fatty liver disease (NAFLD) is responsible for a plethora of disorders leading to the incidence of cardiovascular diseases. In most cases, the cause of NAFLD is not completely understood but has been primarily linked to the occurrence of insulin resistance that often accompanies obesity, dyslipidemia, and Type 2 Diabetes. Hence, it is of paramount importance to elucidate the molecular mechanisms of lipid metabolism underlying the pathogenesis of NAFLD and its comorbidities. Recent evidence indicates the role of lipid turnover in the pathophysiology of NAFLD via autophagy, namely lipophagy. Moreover, certain features of the liver such as its regenerative capacity make it an autophagy-dependent organ. This paper describes several autophagy molecular imaging tools that can be employed to visualize hepatic lipid turnover to elucidate the importance of this signaling cascade in pathological conditions, especially NAFLD.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages185-191
Number of pages7
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1554
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by grants from the National Institutes of Health (DK090364) and the Minnesota Obesity Center (NIH DK050456) to Douglas G. Mashek.

Keywords

  • Autophagy
  • GFP
  • Hepatocytes
  • Immunofluorescence
  • LC3
  • Lipophagy
  • NAFLD
  • Non-alcoholic fatty liver disease
  • Plasmid transfections
  • RFP

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