A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy

Aishwarya Sathyanarayan

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Aberrant liver function as a consequence of excessive fat accumulation as observed in non-alcoholic fatty liver disease (NAFLD) is responsible for a plethora of disorders leading to the incidence of cardiovascular diseases. In most cases, the cause of NAFLD is not completely understood but has been primarily linked to the occurrence of insulin resistance that often accompanies obesity, dyslipidemia, and Type 2 Diabetes. Hence, it is of paramount importance to elucidate the molecular mechanisms of lipid metabolism underlying the pathogenesis of NAFLD and its comorbidities. Recent evidence indicates the role of lipid turnover in the pathophysiology of NAFLD via autophagy, namely lipophagy. Moreover, certain features of the liver such as its regenerative capacity make it an autophagy-dependent organ. This paper describes several autophagy molecular imaging tools that can be employed to visualize hepatic lipid turnover to elucidate the importance of this signaling cascade in pathological conditions, especially NAFLD.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages185-191
Number of pages7
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1554
ISSN (Print)1064-3745

Fingerprint

Immunohistochemistry
Autophagy
Liver
Lipids
Molecular Imaging
Dyslipidemias
Lipid Metabolism
Type 2 Diabetes Mellitus
Insulin Resistance
Comorbidity
Cardiovascular Diseases
Obesity
Fats
Non-alcoholic Fatty Liver Disease
Incidence

Keywords

  • Autophagy
  • GFP
  • Hepatocytes
  • Immunofluorescence
  • LC3
  • Lipophagy
  • NAFLD
  • Non-alcoholic fatty liver disease
  • Plasmid transfections
  • RFP

Cite this

Sathyanarayan, A. (2017). A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy. In Methods in Molecular Biology (pp. 185-191). (Methods in Molecular Biology; Vol. 1554). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6759-9_11

A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy. / Sathyanarayan, Aishwarya.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 185-191 (Methods in Molecular Biology; Vol. 1554).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sathyanarayan, A 2017, A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1554, Humana Press Inc., pp. 185-191. https://doi.org/10.1007/978-1-4939-6759-9_11
Sathyanarayan A. A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 185-191. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6759-9_11
Sathyanarayan, Aishwarya. / A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 185-191 (Methods in Molecular Biology).
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