### Abstract

The tensile stiffness of tissue grown from chondrocyte culture was both measured experimentally and predicted using a composites model theory relating tissue microstructure to macroscopic material stiffness. The tissue was altered by several treatment protocols to provide a wide range of collagen fibril volume fraction (0.015-0.15). The rate of change of tissue modulus with change in collagen volume fraction predicted by the theory was within 14% of the slope of the linear fit through the experimental data, without the use of fitting parameters for the theoretical value of the slope. Use of the model to simulate cytokine mediated tissue digestion suggests that the action of IL-1β and retinoic acid is mainly removal of proteoglycans and some removal of collagen. The model also indicates that the matrix and collagen remaining in the tissue has the same elastic properties as the untreated tissue, and is not damaged due to the alteration. Young's modulus of the collagen fibrils is predicted to be 120 MPa, a value in the range of previous studies. This value is dependent mainly on the matrix modulus and collagen fibril volume fraction and not on Poisson's ratio of either matrix or fibril. Poisson's ratio of the tissue depends primarily on the ratio of the matrix.

Original language | English (US) |
---|---|

Pages (from-to) | 503-509 |

Number of pages | 7 |

Journal | Journal of Biomechanics |

Volume | 32 |

Issue number | 5 |

DOIs | |

State | Published - May 1 1999 |

### Fingerprint

### Keywords

- Articular cartilage
- Cartilage modulus
- Culture tissue
- Osteoarthritis
- Theoretical composites model

### Cite this

*Journal of Biomechanics*,

*32*(5), 503-509. https://doi.org/10.1016/S0021-9290(98)00185-7

**A composites theory predicts the dependence of stiffness of cartilage culture tissues on collagen volume fraction.** / Simha, N. K.; Fedewa, M.; Leo, Perry H; Lewis, Jack L; Oegema, T.

Research output: Contribution to journal › Article

*Journal of Biomechanics*, vol. 32, no. 5, pp. 503-509. https://doi.org/10.1016/S0021-9290(98)00185-7

}

TY - JOUR

T1 - A composites theory predicts the dependence of stiffness of cartilage culture tissues on collagen volume fraction

AU - Simha, N. K.

AU - Fedewa, M.

AU - Leo, Perry H

AU - Lewis, Jack L

AU - Oegema, T.

PY - 1999/5/1

Y1 - 1999/5/1

N2 - The tensile stiffness of tissue grown from chondrocyte culture was both measured experimentally and predicted using a composites model theory relating tissue microstructure to macroscopic material stiffness. The tissue was altered by several treatment protocols to provide a wide range of collagen fibril volume fraction (0.015-0.15). The rate of change of tissue modulus with change in collagen volume fraction predicted by the theory was within 14% of the slope of the linear fit through the experimental data, without the use of fitting parameters for the theoretical value of the slope. Use of the model to simulate cytokine mediated tissue digestion suggests that the action of IL-1β and retinoic acid is mainly removal of proteoglycans and some removal of collagen. The model also indicates that the matrix and collagen remaining in the tissue has the same elastic properties as the untreated tissue, and is not damaged due to the alteration. Young's modulus of the collagen fibrils is predicted to be 120 MPa, a value in the range of previous studies. This value is dependent mainly on the matrix modulus and collagen fibril volume fraction and not on Poisson's ratio of either matrix or fibril. Poisson's ratio of the tissue depends primarily on the ratio of the matrix.

AB - The tensile stiffness of tissue grown from chondrocyte culture was both measured experimentally and predicted using a composites model theory relating tissue microstructure to macroscopic material stiffness. The tissue was altered by several treatment protocols to provide a wide range of collagen fibril volume fraction (0.015-0.15). The rate of change of tissue modulus with change in collagen volume fraction predicted by the theory was within 14% of the slope of the linear fit through the experimental data, without the use of fitting parameters for the theoretical value of the slope. Use of the model to simulate cytokine mediated tissue digestion suggests that the action of IL-1β and retinoic acid is mainly removal of proteoglycans and some removal of collagen. The model also indicates that the matrix and collagen remaining in the tissue has the same elastic properties as the untreated tissue, and is not damaged due to the alteration. Young's modulus of the collagen fibrils is predicted to be 120 MPa, a value in the range of previous studies. This value is dependent mainly on the matrix modulus and collagen fibril volume fraction and not on Poisson's ratio of either matrix or fibril. Poisson's ratio of the tissue depends primarily on the ratio of the matrix.

KW - Articular cartilage

KW - Cartilage modulus

KW - Culture tissue

KW - Osteoarthritis

KW - Theoretical composites model

UR - http://www.scopus.com/inward/record.url?scp=0032924746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032924746&partnerID=8YFLogxK

U2 - 10.1016/S0021-9290(98)00185-7

DO - 10.1016/S0021-9290(98)00185-7

M3 - Article

C2 - 10327004

AN - SCOPUS:0032924746

VL - 32

SP - 503

EP - 509

JO - Journal of Biomechanics

JF - Journal of Biomechanics

SN - 0021-9290

IS - 5

ER -