A composite CMV-IE enhancer/β-actin promoter is ubiquitously expressed in mouse cutaneous epithelium

Janet A. Sawicki, Rebecca J. Morris, Bob Monks, Katsunaga Sakai, Jun Ichi Miyazaki

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable to express a transgene in all the cells of the cutaneous epithelium. Since no ubiquitously expressed promoter sequences had previously been identified, we used lacZ reporter transgenes to test two enhancer/promoter sequences for ubiquitous expression in the skin of adult transgenic mice. We find that a CMV enhancer/CMV promoter is not active in most cell types in the skin, whereas a CMV enhancer/modified β-actin promoter sequence is active in the suprabasal and basal cells of the epidermis as well as in the epithelial cells of the hair follicles, sebaceous glands, and the dermal papillae.

Original languageEnglish (US)
Pages (from-to)367-369
Number of pages3
JournalExperimental Cell Research
Volume244
Issue number1
DOIs
StatePublished - Oct 10 1998
Externally publishedYes

Keywords

  • CMV
  • Cutaneous epithelium
  • Promoter
  • Ubiquitous expression
  • β-actin

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