A comparison of three methods for detecting Candida albicans in patients with Sjögren's syndrome

Nelson L Rhodus, Cynthia Bloomquist, William F Liljemark, Janna Bereuter

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Objective: An immediate chairside technique (Latex Candida) for the detection of Candida albicans was compared with a simple tube culturing technique (Oricult) and the traditional laboratory culturing technique in patients with Sjögren's syndrome. Method and materials: Subjects with primary (n = 9) and secondary (n = 9) Sjögren's syndrome (mean age of 56.7 years; all female) and an age- and sex-matched group of control subjects (n = 9) were selected. Three different methods for culturing Candida albicans were performed for each subject. One culturette was plated on a trypticase soy-agar streptomycin-vancomycin medium plate and incubated for 48 hours at 37°C. Another swab was plated on a reagent paper with the Latex Candida test kit. The third swab was placed in a culture media tube using the Oricult kit and incubated for 48 hours at 37°C. Results: All three techniques indicated a significant difference in the prevalence of Candida between the control group and both Sjögren's groups. The Latex. Candida technique indicated that 78% of all Sjögren's subjects were positive for Candida, while the other two tests indicated that 83% were positive. Conclusion: The Latex Candida technique was comparable to Oricult and streptomycin-vancomycin culturing techniques for negative results and was correctly positive for 90% of cases.

Original languageEnglish (US)
Pages (from-to)107-113
Number of pages7
JournalQuintessence international
Volume29
Issue number2
StatePublished - Feb 1 1998

Fingerprint

Candida albicans
Candida
Latex
Streptomycin
Vancomycin
Agar
Culture Media
Research Design
Control Groups

Keywords

  • Candida albicans
  • Candidiasis
  • Infection
  • Saliva
  • Sjögren's syndrome
  • Xerostomia

Cite this

A comparison of three methods for detecting Candida albicans in patients with Sjögren's syndrome. / Rhodus, Nelson L; Bloomquist, Cynthia; Liljemark, William F; Bereuter, Janna.

In: Quintessence international, Vol. 29, No. 2, 01.02.1998, p. 107-113.

Research output: Contribution to journalReview article

Rhodus, Nelson L ; Bloomquist, Cynthia ; Liljemark, William F ; Bereuter, Janna. / A comparison of three methods for detecting Candida albicans in patients with Sjögren's syndrome. In: Quintessence international. 1998 ; Vol. 29, No. 2. pp. 107-113.
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AB - Objective: An immediate chairside technique (Latex Candida) for the detection of Candida albicans was compared with a simple tube culturing technique (Oricult) and the traditional laboratory culturing technique in patients with Sjögren's syndrome. Method and materials: Subjects with primary (n = 9) and secondary (n = 9) Sjögren's syndrome (mean age of 56.7 years; all female) and an age- and sex-matched group of control subjects (n = 9) were selected. Three different methods for culturing Candida albicans were performed for each subject. One culturette was plated on a trypticase soy-agar streptomycin-vancomycin medium plate and incubated for 48 hours at 37°C. Another swab was plated on a reagent paper with the Latex Candida test kit. The third swab was placed in a culture media tube using the Oricult kit and incubated for 48 hours at 37°C. Results: All three techniques indicated a significant difference in the prevalence of Candida between the control group and both Sjögren's groups. The Latex. Candida technique indicated that 78% of all Sjögren's subjects were positive for Candida, while the other two tests indicated that 83% were positive. Conclusion: The Latex Candida technique was comparable to Oricult and streptomycin-vancomycin culturing techniques for negative results and was correctly positive for 90% of cases.

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