A Comparative Study of Serum Biochemistry, Metabolome and Microbiome Parameters of Clinically Healthy, Normal Weight, Overweight, and Obese Companion Dogs

Genevieve M. Forster, Jonathan Stockman, Noelle Noyes, Adam L. Heuberger, Corey D. Broeckling, Collin M. Bantle, Elizabeth P. Ryan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to compare fecal microbiome, plasma, fecal and urine metabolomes, and serum biochemistry of adult companion dogs according to body condition scores. Blood, serum/plasma, urine, and fecal samples were collected from 66 clinically healthy, adult companion dogs of either normal weight (NW), overweight (OW), or obese dogs (OB). analyses included fecal microbiome analyses via 16S ribosomal RNA gene amplicon; sequencing, nontargeted plasma, fecal, and urine metabolomics using liquid chromatography/gas chromatography-mass; spectrometry, and serum biochemistry for each dog. Few significant differences in serum biochemistry and fecal microbiome Operational Taxonomic Unit (OTU) were found between weight groups and there was high OTU variation between individual dogs. NW dogs had higher relative abundance of the genus Eubacterium (log-fold change 4.3, adjusted P value =.003) and lower relative abundance of the family Bifidobacteriaceae (log-fold change −3.6, adjusted P value =.02) compared to OB dogs. The microbiome of NW dogs had higher OTU richness compared with OB dogs. Metabolome analysis showed 185 plasma, 37 fecal, and 45 urine metabolites that significantly differed between NW and OW or OB dogs. There were notable significant differences in relative abundance of several plasma phospholipid moieties and fecal volatile fatty acids between weight phenotypes. The combinations of host and gut microbiota and metabolic shifts suggest a pattern that could help detection of early metabolic changes in overweight dogs before the development of obesity related disease. The results of this study support the need for continued investigation into sensitive measures of metabolic aberrancies in overweight dogs.

Original languageEnglish (US)
Pages (from-to)126-135
Number of pages10
JournalTopics in Companion Animal Medicine
Volume33
Issue number4
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

Fingerprint

metabolome
Metabolome
Microbiota
blood serum
Biochemistry
biochemistry
Dogs
Weights and Measures
dogs
Serum
urine
Urine
metabolomics
Bifidobacteriaceae
microbiome
obesity-related diseases
Eubacterium (genus)
Eubacterium
16S Ribosomal RNA
Metabolomics

Keywords

  • canine
  • metabolome
  • microbiome
  • obesity

PubMed: MeSH publication types

  • Comparative Study
  • Journal Article

Cite this

A Comparative Study of Serum Biochemistry, Metabolome and Microbiome Parameters of Clinically Healthy, Normal Weight, Overweight, and Obese Companion Dogs. / Forster, Genevieve M.; Stockman, Jonathan; Noyes, Noelle; Heuberger, Adam L.; Broeckling, Corey D.; Bantle, Collin M.; Ryan, Elizabeth P.

In: Topics in Companion Animal Medicine, Vol. 33, No. 4, 01.12.2018, p. 126-135.

Research output: Contribution to journalArticle

Forster, Genevieve M. ; Stockman, Jonathan ; Noyes, Noelle ; Heuberger, Adam L. ; Broeckling, Corey D. ; Bantle, Collin M. ; Ryan, Elizabeth P. / A Comparative Study of Serum Biochemistry, Metabolome and Microbiome Parameters of Clinically Healthy, Normal Weight, Overweight, and Obese Companion Dogs. In: Topics in Companion Animal Medicine. 2018 ; Vol. 33, No. 4. pp. 126-135.
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