Abstract
A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 239-245 |
| Number of pages | 7 |
| Journal | Journal of Immunological Methods |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 1977 |
Bibliographical note
Funding Information:This work was supported by NIH USPHS Grants AI-12478-01 and HL-06314-15, and by a grant from the Minnesota Arthritis Foundation.
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