A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation

Maren L Mahowald; Barry S. Handwerger; Erskine M. Capertone; Steven D. Douglas

doi:10.1016/0022-1759(77)90061-8

A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation

Maren L Mahowald, Barry S. Handwerger, Erskine M. Capertone, Steven D. Douglas

Medicine - Veteran's Administration Medical Center

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.

Original language	English (US)
Pages (from-to)	239-245
Number of pages	7
Journal	Journal of Immunological Methods
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(77)90061-8
State	Published - Apr 1977

Bibliographical note

Funding Information:
This work was supported by NIH USPHS Grants AI-12478-01 and HL-06314-15, and by a grant from the Minnesota Arthritis Foundation.

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10.1016/0022-1759(77)90061-8

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title = "A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation",

abstract = "A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.",

author = "Mahowald, {Maren L} and Handwerger, {Barry S.} and Capertone, {Erskine M.} and Douglas, {Steven D.}",

note = "Funding Information: This work was supported by NIH USPHS Grants AI-12478-01 and HL-06314-15, and by a grant from the Minnesota Arthritis Foundation.",

year = "1977",

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doi = "10.1016/0022-1759(77)90061-8",

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AU - Mahowald, Maren L

AU - Handwerger, Barry S.

AU - Capertone, Erskine M.

AU - Douglas, Steven D.

N1 - Funding Information: This work was supported by NIH USPHS Grants AI-12478-01 and HL-06314-15, and by a grant from the Minnesota Arthritis Foundation.

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N2 - A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.

AB - A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.

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A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation

Abstract

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