TY - JOUR
T1 - A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation
AU - Mahowald, Maren L
AU - Handwerger, Barry S.
AU - Capertone, Erskine M.
AU - Douglas, Steven D.
N1 - Funding Information:
This work was supported by NIH USPHS Grants AI-12478-01 and HL-06314-15, and by a grant from the Minnesota Arthritis Foundation.
PY - 1977/4
Y1 - 1977/4
N2 - A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.
AB - A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.
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U2 - 10.1016/0022-1759(77)90061-8
DO - 10.1016/0022-1759(77)90061-8
M3 - Article
C2 - 404361
AN - SCOPUS:0017614920
VL - 15
SP - 239
EP - 245
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 3
ER -