A Cell Proliferation and Inflammatory Signature Is Induced by Lawsonia intracellularis Infection in Swine

Fernando L. Leite, Juan E Abrahante - Llorens, Erika Vasquez, Fabio A Vannucci, Connie J Gebhart, Nathan Winkelman, Adam Mueller, Jerry L Torrison, Zachary Rambo, Richard E. Isaacson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Lawsonia intracellularis causes porcine proliferative enteropathy. This is an enteric disease characterized by thickening of the wall of the ileum that leads to decreased growth of animals and diarrhea. In this study, we investigated the host response to L. intracellularis infection by performing transcriptomic and pathway analysis of intestinal tissue samples from groups of infected and noninfected animals at 14, 21, and 28 days postchallenge. At the peak of infection, when animals developed the most severe lesions, infected animals had higher levels of several gene transcripts involved in cellular proliferation and inflammation, including matrix metalloproteinase-7 (MMP7), transglutaminase-2 (TGM2), and oncostatin M (OSM). Histomorphology also revealed general features of intestinal inflammation. This study identified important pathways associated with the host response in developing and resolving lesions due to L. intracellularis infection.IMPORTANCELawsonia intracellularis is among the most important enteric pathogens of swine, and it can also infect other mammalian species. Much is still unknown regarding its pathogenesis and the host response, especially at the site of infection. In this study, we uncovered several novel genes and pathways associated with infection. Differentially expressed transcripts, in addition to histological changes in infected tissue, revealed striking similarities between L. intracellularis infection and cellular proliferation mechanisms described in some cancers and inflammatory diseases of the gastrointestinal tract. This research sheds important light into the pathogenesis of L. intracellularis and the host response associated with the lesions caused by infection.

Original languageEnglish (US)
JournalmBio
Volume10
Issue number1
DOIs
StatePublished - Jan 29 2019

Fingerprint

Desulfovibrionaceae Infections
Lawsonia Bacteria
Swine
Cell Proliferation
Infection
Oncostatin M
Matrix Metalloproteinase 7
Inflammation
Ileum
Genes
Gastrointestinal Tract
Diarrhea
Growth
Research

Keywords

  • cell proliferation
  • host response
  • intestinal inflammation
  • Lawsonia intracellularis
  • transcriptome

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

A Cell Proliferation and Inflammatory Signature Is Induced by Lawsonia intracellularis Infection in Swine. / Leite, Fernando L.; Abrahante - Llorens, Juan E; Vasquez, Erika; Vannucci, Fabio A; Gebhart, Connie J; Winkelman, Nathan; Mueller, Adam; Torrison, Jerry L; Rambo, Zachary; Isaacson, Richard E.

In: mBio, Vol. 10, No. 1, 29.01.2019.

Research output: Contribution to journalArticle

Leite, Fernando L. ; Abrahante - Llorens, Juan E ; Vasquez, Erika ; Vannucci, Fabio A ; Gebhart, Connie J ; Winkelman, Nathan ; Mueller, Adam ; Torrison, Jerry L ; Rambo, Zachary ; Isaacson, Richard E. / A Cell Proliferation and Inflammatory Signature Is Induced by Lawsonia intracellularis Infection in Swine. In: mBio. 2019 ; Vol. 10, No. 1.
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