A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes

Raffaele Hellweg, Ashley Mooneyham, Martina Bazzaro

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Here we describe a method for identifying genes and genetic pathways responsible for chemoresistance in cancer cells. The method is based on generation and characterization of matched pairs of chemotherapy-sensitive/chemotherapy-resistant cancer cell lines. In this protocol we are using endometrial cancer cell lines treated with carboplatin and paclitaxel, which are first-line chemotherapies for gynecologic malignancies. The chemoresistant cells and their chemosensitive counterparts are used for downstream applications including bulk RNA-sequencing analysis to identify a set of genes and pathways that are associated with chemoresistance. Identification of pathways responsible for innate or acquired chemoresistance is of paramount importance for the identification of biomarkers for cancer risk stratification and prognosis, and as a pharmacogenomics model for identification of alternative chemotherapy approaches for treatment of patients with recurrent and chemoresistant disease.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-90
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1907
ISSN (Print)1064-3745

Fingerprint

Neoplasm Genes
Drug Therapy
RNA Sequence Analysis
Cell Line
Neoplasms
Pharmacogenetics
Carboplatin
Endometrial Neoplasms
Tumor Biomarkers
Paclitaxel
Genes
Therapeutics

Keywords

  • Endometrial cancer
  • In vitro chemoresistance
  • Platinum resistance
  • Taxane resistance

PubMed: MeSH publication types

  • Journal Article

Cite this

Hellweg, R., Mooneyham, A., & Bazzaro, M. (2019). A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes. In Methods in Molecular Biology (pp. 83-90). (Methods in Molecular Biology; Vol. 1907). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8967-6_6

A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes. / Hellweg, Raffaele; Mooneyham, Ashley; Bazzaro, Martina.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 83-90 (Methods in Molecular Biology; Vol. 1907).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hellweg, R, Mooneyham, A & Bazzaro, M 2019, A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1907, Humana Press Inc., pp. 83-90. https://doi.org/10.1007/978-1-4939-8967-6_6
Hellweg R, Mooneyham A, Bazzaro M. A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 83-90. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8967-6_6
Hellweg, Raffaele ; Mooneyham, Ashley ; Bazzaro, Martina. / A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 83-90 (Methods in Molecular Biology).
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