A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes

Raffaele Hellweg, Ashley Mooneyham, Martina Bazzaro

Research output: Contribution to journalArticle

Abstract

Here we describe a method for identifying genes and genetic pathways responsible for chemoresistance in cancer cells. The method is based on generation and characterization of matched pairs of chemotherapy-sensitive/chemotherapy-resistant cancer cell lines. In this protocol we are using endometrial cancer cell lines treated with carboplatin and paclitaxel, which are first-line chemotherapies for gynecologic malignancies. The chemoresistant cells and their chemosensitive counterparts are used for downstream applications including bulk RNA-sequencing analysis to identify a set of genes and pathways that are associated with chemoresistance. Identification of pathways responsible for innate or acquired chemoresistance is of paramount importance for the identification of biomarkers for cancer risk stratification and prognosis, and as a pharmacogenomics model for identification of alternative chemotherapy approaches for treatment of patients with recurrent and chemoresistant disease.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalMethods in Molecular Biology
Volume1907
DOIs
StatePublished - Jan 1 2019

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Neoplasm Genes
Drug Therapy
RNA Sequence Analysis
Cell Line
Neoplasms
Pharmacogenetics
Carboplatin
Endometrial Neoplasms
Tumor Biomarkers
Paclitaxel
Genes
Therapeutics

Keywords

  • Endometrial cancer
  • In vitro chemoresistance
  • Platinum resistance
  • Taxane resistance

PubMed: MeSH publication types

  • Journal Article

Cite this

A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes. / Hellweg, Raffaele; Mooneyham, Ashley; Bazzaro, Martina.

In: Methods in Molecular Biology, Vol. 1907, 01.01.2019, p. 83-90.

Research output: Contribution to journalArticle

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