A boundary element method/Brownian dynamics approach for simulating DNA electrophoresis in electrically insulating microfabricated devices

Jaeseol Cho, Martin Kenward, Kevin D. Dorfman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We present an approach for merging boundary element method (BEM) solutions of the electric field in electrically insulating complex geometries with Brownian dynamics (BD) simulations of DNA electrophoresis therein. Although a rote application of the standard BEM algorithm proves inaccurate and prohibitively expensive, we show that regularization of the near-wall electric field and an updating scheme commensurate with the characteristic length scale of the BD simulation furnishes a robust, efficient simulation protocol. The accuracy of the BEM-BD method is verified by simulating λ-DNA collisions with an isolated, insulating cylindrical obstacle and comparing the results with equivalent BD simulations that employ the exact solution for the electric field. The computational overhead of our implementation of BEM-BD is comparable to an existing finite element method/BD approach. The BEM-BD algorithm is readily parallelized and well-suited to time-dependent and responsive electric fields, making it broadly applicable to simulating DNA electrophoresis in microfluidic devices.

Original languageEnglish (US)
Pages (from-to)1482-1489
Number of pages8
JournalELECTROPHORESIS
Volume30
Issue number9
DOIs
StatePublished - May 1 2009

Keywords

  • Boundary element method
  • Brownian dynamics
  • Microfluidics
  • Modeling
  • Simulation

Fingerprint

Dive into the research topics of 'A boundary element method/Brownian dynamics approach for simulating DNA electrophoresis in electrically insulating microfabricated devices'. Together they form a unique fingerprint.

Cite this