A 'blue' copper oxidase from Nitrosomonas europaea

Alan A. DiSpirito, Larry R. Taaffe, John D Lipscomb, Alan B. Hooper

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A soluble copper-containing protein with p-phenylenediamine oxidase activity was purified from Nitrosomonas europaea by flat-bed isoelectric focusing and chromatography on Sephacryl S-300. The native and subunit molecular weights were 127 500 and 40 100, respectively; the isoelectric point was pH 4.63. The protein had an absorption maximum at 607 nm in the oxidized form with an extinction coefficient of 6.0 cm-1 · mM-1 at pH 7.5. On reduction with dithionite, the absorbance was abolished. The electron paramagnetic resonance spectrum of the protein showed evidence for both Type 1 and Type 2 copper in a 1:1 ratio. The protein catalyzed the aerobic oxidation of p-phenylenediamine, cytochrome c-554, and hydroxylamine oxidoreductase. The enzyme catalyzed the reduction of nitrite with cytochrome c-552 as electron donor. In contrast, p-phenylenediamine, reduced cytochrome c-554, and hydroxylamine oxidoreductase were not active as electron donors for nitrite reduction.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume827
Issue number3
DOIs
StatePublished - Mar 1 1985

Fingerprint

Nitrosomonas europaea
hydroxylamine dehydrogenase
Cytochromes c
Nitrites
Copper
Proteins
Electrons
Dithionite
Electron Spin Resonance Spectroscopy
Isoelectric Point
Isoelectric Focusing
Chromatography
Paramagnetic resonance
Molecular Weight
Molecular weight
Oxidation
copper oxidase
Enzymes

Keywords

  • (N. europaea)
  • Blue copper oxidase
  • Copper protein
  • ESR

Cite this

A 'blue' copper oxidase from Nitrosomonas europaea. / DiSpirito, Alan A.; Taaffe, Larry R.; Lipscomb, John D; Hooper, Alan B.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 827, No. 3, 01.03.1985, p. 320-326.

Research output: Contribution to journalArticle

DiSpirito, Alan A. ; Taaffe, Larry R. ; Lipscomb, John D ; Hooper, Alan B. / A 'blue' copper oxidase from Nitrosomonas europaea. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1985 ; Vol. 827, No. 3. pp. 320-326.
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