A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

Emiliano Salvagni; Geoffrey Berguig; Elisabeth Engel; J. Carlos Rodriguez-Cabello; Geraldine Coullerez; Marcus Textor; Josep A. Planell; F. Javier Gil; Conrado Aparicio

doi:10.1016/j.colsurfb.2013.10.008

A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

Emiliano Salvagni, Geoffrey Berguig, Elisabeth Engel, J. Carlos Rodriguez-Cabello, Geraldine Coullerez, Marcus Textor, Josep A. Planell, F. Javier Gil, Conrado Aparicio

Restorative Sciences

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9ng/cm²) compared to the bare metal surface (310ng/cm²). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.

Original language	English (US)
Pages (from-to)	225-233
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	114
DOIs	https://doi.org/10.1016/j.colsurfb.2013.10.008
State	Published - Feb 1 2014

Bibliographical note

Funding Information:
We would like to thank Melba Navarro for helping with the cell in vitro studies. This work was partially funded by the Commission of the European Communities: Marie Curie Research Training Network BioPolySur-MRTN-CT-2004 005516f , the European Commission through the 7th Framework Programme: projects HEALTH-F4-2011-278557 and NMP3-LA-2011-263363 , the Spanish Ministry of Science and Innovation : project MAT 2008-06887-C03-03 and Juan de la Cierva grant JCI-2008-2940 . The Spanish Minister of Economy and Competitiveness-MEC : projects MAT2009-14195-C03-03 , MAT-2010-15982 , MAT2010-15310 , IT2009-0089 , ACI2009-0890 , and PRI-PIBAR-2011-1403 , the Junta de Castilla y León-JCyL : projects VA034A09 and VA0049A11-2 . CIBER-BBN project: CB06-01-1038, Junta de Castilla y León and the Instituto de Salud Carlos III under the Network Center of Regenerative Medicine and Cellular Therapy of Castilla and León , and the Office of the Vice President for Research of the University of Minnesota , Grant-in-Aid of Research, Artistry and Scholarship 55466 .

Keywords

Bioactivity
Biofunctionalization
Biomimetic
Cell response
Protein immobilization
Protein resistance

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10.1016/j.colsurfb.2013.10.008

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title = "A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces",

abstract = "We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9ng/cm2) compared to the bare metal surface (310ng/cm2). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.",

keywords = "Bioactivity, Biofunctionalization, Biomimetic, Cell response, Protein immobilization, Protein resistance",

author = "Emiliano Salvagni and Geoffrey Berguig and Elisabeth Engel and Rodriguez-Cabello, {J. Carlos} and Geraldine Coullerez and Marcus Textor and Planell, {Josep A.} and Gil, {F. Javier} and Conrado Aparicio",

note = "Funding Information: We would like to thank Melba Navarro for helping with the cell in vitro studies. This work was partially funded by the Commission of the European Communities: Marie Curie Research Training Network BioPolySur-MRTN-CT-2004 005516f , the European Commission through the 7th Framework Programme: projects HEALTH-F4-2011-278557 and NMP3-LA-2011-263363 , the Spanish Ministry of Science and Innovation : project MAT 2008-06887-C03-03 and Juan de la Cierva grant JCI-2008-2940 . The Spanish Minister of Economy and Competitiveness-MEC : projects MAT2009-14195-C03-03 , MAT-2010-15982 , MAT2010-15310 , IT2009-0089 , ACI2009-0890 , and PRI-PIBAR-2011-1403 , the Junta de Castilla y Le{\'o}n-JCyL : projects VA034A09 and VA0049A11-2 . CIBER-BBN project: CB06-01-1038, Junta de Castilla y Le{\'o}n and the Instituto de Salud Carlos III under the Network Center of Regenerative Medicine and Cellular Therapy of Castilla and Le{\'o}n , and the Office of the Vice President for Research of the University of Minnesota , Grant-in-Aid of Research, Artistry and Scholarship 55466 .",

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T1 - A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

AU - Salvagni, Emiliano

AU - Berguig, Geoffrey

AU - Engel, Elisabeth

AU - Rodriguez-Cabello, J. Carlos

AU - Coullerez, Geraldine

AU - Textor, Marcus

AU - Planell, Josep A.

AU - Gil, F. Javier

AU - Aparicio, Conrado

N1 - Funding Information: We would like to thank Melba Navarro for helping with the cell in vitro studies. This work was partially funded by the Commission of the European Communities: Marie Curie Research Training Network BioPolySur-MRTN-CT-2004 005516f , the European Commission through the 7th Framework Programme: projects HEALTH-F4-2011-278557 and NMP3-LA-2011-263363 , the Spanish Ministry of Science and Innovation : project MAT 2008-06887-C03-03 and Juan de la Cierva grant JCI-2008-2940 . The Spanish Minister of Economy and Competitiveness-MEC : projects MAT2009-14195-C03-03 , MAT-2010-15982 , MAT2010-15310 , IT2009-0089 , ACI2009-0890 , and PRI-PIBAR-2011-1403 , the Junta de Castilla y León-JCyL : projects VA034A09 and VA0049A11-2 . CIBER-BBN project: CB06-01-1038, Junta de Castilla y León and the Instituto de Salud Carlos III under the Network Center of Regenerative Medicine and Cellular Therapy of Castilla and León , and the Office of the Vice President for Research of the University of Minnesota , Grant-in-Aid of Research, Artistry and Scholarship 55466 .

PY - 2014/2/1

Y1 - 2014/2/1

N2 - We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9ng/cm2) compared to the bare metal surface (310ng/cm2). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.

AB - We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9ng/cm2) compared to the bare metal surface (310ng/cm2). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.

KW - Bioactivity

KW - Biofunctionalization

KW - Biomimetic

KW - Cell response

KW - Protein immobilization

KW - Protein resistance

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SN - 0927-7765

VL - 114

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

Abstract

Bibliographical note

Keywords

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