We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9ng/cm2) compared to the bare metal surface (310ng/cm2). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.
Bibliographical noteFunding Information:
We would like to thank Melba Navarro for helping with the cell in vitro studies. This work was partially funded by the Commission of the European Communities: Marie Curie Research Training Network BioPolySur-MRTN-CT-2004 005516f , the European Commission through the 7th Framework Programme: projects HEALTH-F4-2011-278557 and NMP3-LA-2011-263363 , the Spanish Ministry of Science and Innovation : project MAT 2008-06887-C03-03 and Juan de la Cierva grant JCI-2008-2940 . The Spanish Minister of Economy and Competitiveness-MEC : projects MAT2009-14195-C03-03 , MAT-2010-15982 , MAT2010-15310 , IT2009-0089 , ACI2009-0890 , and PRI-PIBAR-2011-1403 , the Junta de Castilla y León-JCyL : projects VA034A09 and VA0049A11-2 . CIBER-BBN project: CB06-01-1038, Junta de Castilla y León and the Instituto de Salud Carlos III under the Network Center of Regenerative Medicine and Cellular Therapy of Castilla and León , and the Office of the Vice President for Research of the University of Minnesota , Grant-in-Aid of Research, Artistry and Scholarship 55466 .
- Cell response
- Protein immobilization
- Protein resistance