A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene

D. Danesh, S. Peñuela, J. Mudge, R. L. Denny, H. Nordstrom, J. P. Martinez, N. D. Young

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

We constructed a bacterial artificial chromosome (BAC) library for soybean (Glycine max) consisting of approximately 30000 clones with an average insert size of 120 kilobase pairs. The library was successfully screened with restriction fragment length polymorphism (RFLP) and microsatellite markers tightly linked to a major resistance gene for the cyst nematode, Heterodera glycines. Since many soybean RFLPs hybridize to duplicate loci, BACs homologous to duplicate RFLP loci were distinguished by digestion with the restriction enzyme originally used to map the RFLP, followed by a comparison of the hybridizing fragments. Linkage mapping of BAC clones identified with markers linked to the cyst nematode resistance gene demonstrated that these clones were located at the expected chromosomal positions and that there were no indications of chimeras within the genomic inserts.

Original languageEnglish (US)
Pages (from-to)196-202
Number of pages7
JournalTheoretical and Applied Genetics
Volume96
Issue number2
DOIs
StatePublished - Feb 1 1998

Keywords

  • Chromosome walking
  • Gene mapping
  • Glycine max
  • Heterodera glycines
  • High-molecular-weight DNA
  • Positional cloning

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