A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice

E. J. Conley, V. Nduati, J. L. Gonzalez-Hernandez, A. Mesfin, M. Trudeau-Spanjers, S. Chao, G. R. Lazo, D. D. Hummel, O. D. Anderson, L. L. Qi, B. S. Gill, B. Echalier, A. M. Linkiewicz, J. Dubcovsky, E. D. Akhunov, J. Dvořák, J. H. Peng, N. L.V. Lapitan, M. S. Pathan, H. T. NguyenX. F. Ma, Miftahudin, J. P. Gustafson, R. A. Greene, M. E. Sorrells, K. G. Hossain, V. Kalavacharla, S. F. Kianian, D. Sidhu, M. Dilbirligi, K. S. Gill, D. W. Choi, R. D. Fenton, T. J. Close, P. E. McGuire, C. O. Qualset, J. A. Anderson

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67 Scopus citations

Abstract

The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7.

Original languageEnglish (US)
Pages (from-to)625-637
Number of pages13
JournalGenetics
Volume168
Issue number2
DOIs
StatePublished - Oct 2004

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