Abstract
Structure–property relationships between polymers and their ability to interact with nucleic acids such as oligonucleotides, small interfering RNAs, and plasmid DNAs in order to deliver them into cells in culture and in mammals are described. The structure of the polymers has a strong influence on their ability to bind to nucleic acids and function as delivery agents. Numerous structural features are illustrated and their effects on nucleic acid delivery into cultured cells are described. The features necessary for systemic delivery of nucleic acids in mammals are outlined, and examples of polymer delivery systems and their function in mice are illustrated. Finally, the very few examples of polymer-based delivery of nucleic acids in humans are presented.
| Original language | English (US) |
|---|---|
| Title of host publication | Polymer Science |
| Subtitle of host publication | a Comprehensive Reference: Volume 1-10 |
| Publisher | Elsevier |
| Pages | 497-527 |
| Number of pages | 31 |
| Volume | 1-10 |
| ISBN (Electronic) | 9780080878621 |
| DOIs | |
| State | Published - Jan 1 2012 |
Bibliographical note
Funding Information:Theresa M. Reineke is an associate professor of chemistry at the Virginia Tech. She is a materials chemist with research interests in the design and development of macromolecules for the delivery of nucleic acids and diagnostic imaging. After receiving a PhD from The University of Michigan and an NIH Postdoctoral Fellowship at the California Institute of Technology, she joined the faculty at the University of Cincinnati as an assistant professor in 2002. In 2008, she relocated her research program and group to Virginia Tech as an associate professor of chemistry. Professor Reineke has won numerous awards including the ACS PMSE Division Arthur K. Doolittle Award, Beckman Young Investigator Award, NSF CAREER Award, Alfred P. Sloan Fellowship, Camille Dreyfus Teacher-Scholar Award, and NIH Director’s New Innovator Award. Professor Reineke serves as the Chair of the Molecular Conjugates Committee of the American Society of Cell and Gene Therapy, an American Chemical Society POLY Division Councilor, on the Advisory Boards of Macromolecular Bioscience and Bioconjugate Chemistry , and on the Scientific Advisory Board for Techulon, Inc. Techulon Inc. was founded based on Professor Reineke’s technology and is currently marketing her polymeric nucleic acid delivery vehicles. Further information about the Reineke Research Group can be found at www.reinekegroup.org .
Publisher Copyright:
© 2012 Elsevier B.V. All rights reserved.
Keywords
- Clinical trials
- DNA
- Delivery
- Nucleic acids
- Polycations
- siRNA