[6]-Shogaol inhibits growth and induces apoptosis of non-small cell lung cancer cells by directly regulating Akt1/2

Myoung Ok Kim, Mee Hyun Lee, Naomi Hamada, Sung Hyun Kim, KiBeom Bae, Zunnan Huang, Dong Joon Kim, Srinivasa Reddy Kanamata Reddy, Sung-Young Lee, Si Jun Park, Jae Young Kim, Hua Xie, Joydeb Kumar Kundu, Zae Young Ryoo, Ann M. Bode, Young Joon Surh, Zigang Dong

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Non-small cell lung cancer (NSCLC) is the leading cause of cancer mortality worldwide. Despite progress in developing chemotherapeutics for the treatment of NSCLC, primary and secondary resistance limits therapeutic success. NSCLC cells exhibit multiple mutations in the epidermal growth factor receptor (EGFR), which cause aberrant activation of diverse cell signaling pathways. Therefore, suppression of the inappropriate amplification of EGFR downstream signaling cascadesis considered to be a rational therapeutic and preventivestrategy for the management of NSCLC. Our initial molecular target-oriented virtual screening revealed that the ginger components,including [6]-shogaol, [6]-paradol and [6]-gingerol, seem to be potential candidates for the prevention and treatment of NSCLC. Among the compounds, [6]-shogaol showed the greatest inhibitory effects on the NSCLC cell proliferation and anchorage-independent growth. [6]-Shogaol induced cell cycle arrest (G1 or G2/M) and apoptosis. Furthermore, [6]-shogaol inhibited Akt kinase activity, a downstream mediator of EGFR signaling, by binding with an allosteric site of Akt. In NCI-H1650 lung cancer cells, [6]-shogaol reduced the constitutive phosphorylation of signal transducer and activator of transcription-3 (STAT3) and decreased the expression of cyclinD1/3, which are target proteins in the Akt signaling pathway.The induction of apoptosis in NCI-H1650 cells by [6]-shogaolcorresponded with the cleavage of caspase-3 and caspase-7.Moreover, intraperitoneal administration of [6]-shogaol inhibitedthe growth of NCI-H1650 cells as tumor xenografts in nudemice. [6]-Shogaol suppressed the expression of Ki-67, cyclin D1 and phosphorylated Akt and STAT3 and increased terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positivity in xenograft tumors. The current study clearly indicates that [6]-shogaol can be exploited for the prevention and/or treatment of NSCLC.

Original languageEnglish (US)
Pages (from-to)683-691
Number of pages9
JournalCarcinogenesis
Volume35
Issue number3
DOIs
StatePublished - Mar 2014

Fingerprint Dive into the research topics of '[6]-Shogaol inhibits growth and induces apoptosis of non-small cell lung cancer cells by directly regulating Akt1/2'. Together they form a unique fingerprint.

  • Cite this

    Kim, M. O., Lee, M. H., Hamada, N., Kim, S. H., Bae, K., Huang, Z., Kim, D. J., Kanamata Reddy, S. R., Lee, S-Y., Park, S. J., Kim, J. Y., Xie, H., Kundu, J. K., Ryoo, Z. Y., Bode, A. M., Surh, Y. J., & Dong, Z. (2014). [6]-Shogaol inhibits growth and induces apoptosis of non-small cell lung cancer cells by directly regulating Akt1/2. Carcinogenesis, 35(3), 683-691. https://doi.org/10.1093/carcin/bgt365