5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

P. G. Siliciano, C. Guthrie

Research output: Contribution to journalArticle

201 Scopus citations

Abstract

Using a strategy of compensatory nucleotide changes between yeast U1 and a 5' splice site, we have analyzed the contribution of base-pairing to the efficiency and fidelity of pre-mRNA splicing in vivo. Watson-Crick base-pairing interactions with U1 can be demonstrated at intron positions 1 and 5 but not at position 4. Moreover, restoration of the ability to pair with U1 is not sufficient to restore activity in the second step of splicing to intron position 1 mutants. Finally, in contrast to recent observations in mammalian systems, we find that the precise position of 5' splice site cleavage is not determined solely by the base-pairing interaction with U1. Rather, the presence of a G residue at position 5 is required for the correct localization of the nucleolytic event. Taken together, these results indicate that the demands for 5' splice site selection and utilization are more complex than a simple maximization of Watson-Crick interactions with U1.

Original languageEnglish (US)
Pages (from-to)1258-1267
Number of pages10
JournalGenes & development
Volume2
Issue number10
DOIs
StatePublished - Oct 1988
Externally publishedYes

Fingerprint Dive into the research topics of '5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.'. Together they form a unique fingerprint.

  • Cite this