TY - JOUR
T1 - 5-Aminothiophene-2,4-dicarboxamide analogues as hepatitis B virus capsid assembly effectors
AU - Tang, Jing
AU - Huber, Andrew D.
AU - Pineda, Dallas L.
AU - Boschert, Kelsey N.
AU - Wolf, Jennifer J.
AU - Kankanala, Jayakanth
AU - Xie, Jiashu
AU - Sarafianos, Stefan G.
AU - Wang, Zhengqiang
N1 - Funding Information:
This research was supported by the National Institutes of Health ( R01AI121315 to SGS and ZW).
Publisher Copyright:
© 2018 Elsevier Masson SAS
PY - 2019/2/15
Y1 - 2019/2/15
N2 - Chronic hepatitis B virus (HBV) infection represents a major health threat. Current FDA-approved drugs do not cure HBV. Targeting HBV core protein (Cp) provides an attractive approach toward HBV inhibition and possibly infection cure. We have previously identified and characterized a 5-amino-3-methylthiophene-2,4-dicarboxamide (ATDC) compound as a structurally novel hit for capsid assembly effectors (CAEs). We report herein hit validation through studies on absorption, distribution, metabolism and excretion (ADME) properties and pharmacokinetics (PK), and hit optimization via analogue synthesis aiming to probe the structure-activity relationship (SAR) and structure-property relationship (SPR). In the end, these medicinal chemistry efforts led to the identification of multiple analogues strongly binding to Cp, potently inhibiting HBV replication in nanomolar range without cytotoxicity, and exhibiting good oral bioavailability (F). Two of our analogues, 19o (EC50 = 0.11 μM, CC50 > 100 μM, F = 25%) and 19k (EC50 = 0.31 μM, CC50 > 100 μM, F = 46%), displayed overall lead profiles superior to reported CAEs 7–10 used in our studies.
AB - Chronic hepatitis B virus (HBV) infection represents a major health threat. Current FDA-approved drugs do not cure HBV. Targeting HBV core protein (Cp) provides an attractive approach toward HBV inhibition and possibly infection cure. We have previously identified and characterized a 5-amino-3-methylthiophene-2,4-dicarboxamide (ATDC) compound as a structurally novel hit for capsid assembly effectors (CAEs). We report herein hit validation through studies on absorption, distribution, metabolism and excretion (ADME) properties and pharmacokinetics (PK), and hit optimization via analogue synthesis aiming to probe the structure-activity relationship (SAR) and structure-property relationship (SPR). In the end, these medicinal chemistry efforts led to the identification of multiple analogues strongly binding to Cp, potently inhibiting HBV replication in nanomolar range without cytotoxicity, and exhibiting good oral bioavailability (F). Two of our analogues, 19o (EC50 = 0.11 μM, CC50 > 100 μM, F = 25%) and 19k (EC50 = 0.31 μM, CC50 > 100 μM, F = 46%), displayed overall lead profiles superior to reported CAEs 7–10 used in our studies.
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U2 - 10.1016/j.ejmech.2018.12.047
DO - 10.1016/j.ejmech.2018.12.047
M3 - Article
C2 - 30594676
AN - SCOPUS:85059147386
SN - 0223-5234
VL - 164
SP - 179
EP - 192
JO - CHIM.THER.
JF - CHIM.THER.
ER -
