4-Hydroxyphenylpyruvate dioxygenase is an iron-tyrosinate protein

F. C. Bradley, S. Lindstedt, John D Lipscomb, Larry Que, A. L. Roe, M. Rundgren

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A resonance Raman investigation into the blue chromophore of 4-hydroxyphenylpyruvate dioxygenase, a non-heme iron enzyme from Pseudomonas P. J. 874, reveals the presence of enhanced vibrations characteristic of tyrosinate coordination to the iron center. The excitation profiles for these features show that they are associated with the 595 nm absorption feature. EPR studies of this enzyme indicate the presence of a high-spin ferric center in a rhombic environment, as evidenced by a signal at g = 4.3 with the correct intensity for the measured iron content. This enzyme thus belongs to the emerging class of iron-tyrosinate proteins.

Original languageEnglish (US)
Pages (from-to)11693-11696
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number25
StatePublished - Dec 1 1986

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4-Hydroxyphenylpyruvate Dioxygenase
Iron
Proteins
Enzymes
Chromophores
Vibration
Pseudomonas
Paramagnetic resonance

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Bradley, F. C., Lindstedt, S., Lipscomb, J. D., Que, L., Roe, A. L., & Rundgren, M. (1986). 4-Hydroxyphenylpyruvate dioxygenase is an iron-tyrosinate protein. Journal of Biological Chemistry, 261(25), 11693-11696.

4-Hydroxyphenylpyruvate dioxygenase is an iron-tyrosinate protein. / Bradley, F. C.; Lindstedt, S.; Lipscomb, John D; Que, Larry; Roe, A. L.; Rundgren, M.

In: Journal of Biological Chemistry, Vol. 261, No. 25, 01.12.1986, p. 11693-11696.

Research output: Contribution to journalArticle

Bradley, FC, Lindstedt, S, Lipscomb, JD, Que, L, Roe, AL & Rundgren, M 1986, '4-Hydroxyphenylpyruvate dioxygenase is an iron-tyrosinate protein', Journal of Biological Chemistry, vol. 261, no. 25, pp. 11693-11696.
Bradley FC, Lindstedt S, Lipscomb JD, Que L, Roe AL, Rundgren M. 4-Hydroxyphenylpyruvate dioxygenase is an iron-tyrosinate protein. Journal of Biological Chemistry. 1986 Dec 1;261(25):11693-11696.
Bradley, F. C. ; Lindstedt, S. ; Lipscomb, John D ; Que, Larry ; Roe, A. L. ; Rundgren, M. / 4-Hydroxyphenylpyruvate dioxygenase is an iron-tyrosinate protein. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 25. pp. 11693-11696.
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