4-Cyanoindole-2′-deoxyribonucleoside (4CIN): A Universal Fluorescent Nucleoside Analogue

Kellan T. Passow, Daniel A. Harki

Research output: Contribution to journalArticle

5 Scopus citations


The synthesis and characterization of a universal and fluorescent nucleoside, 4-cyanoindole-2′-deoxyribonucleoside (4CIN), and its incorporation into DNA is described. 4CIN is a highly efficient fluorophore with quantum yields >0.90 in water. When incorporated into duplex DNA, 4CIN pairs equivalently with native nucleobases and has uniquely high quantum yields ranging from 0.15 to 0.31 depending on sequence and hybridization contexts, surpassing that of 2-aminopurine, the prototypical nucleoside fluorophore. 4CIN constitutes a new isomorphic nucleoside for diverse applications.

Original languageEnglish (US)
Pages (from-to)4310-4313
Number of pages4
JournalOrganic Letters
Issue number14
StatePublished - Jul 20 2018

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