4-chloroindole-3-acetic and indole-3-acetic acids in Pisum sativum

Volker Magnus, Jocelyn A. Ozga, Dennis M. Reinecke, Gerald L. Pierson, Thomas A. Larue, Jerry D. Cohen, Mark L. Brenner

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42 Scopus citations

Abstract

Endogenous IAA and 4-chloroindole-3-acetic acid (4-CI-IAA) were analysed in vegetative and reproductive tissues of the garden pea (Pisum sativum) using GC-MS selected ion monitoring in the presence of stable-isotope labelled internal standards. In fruit collected 3-8 days after anthesis (DAA) conjugates of both auxins were more abundant than the free hormones. Auxin levels (ng g-1 fr. wt) in the seeds were higher by 1-2 orders of magnitude than in the pericarps. However, as seeds are small at this stage, the pericarp nevertheless contains a substantial fraction of the overall quantity of IAA and 4-CI-IAA. While, in young fruit tissues, IAA was more abundant than 4-CI-IAA, the opposite was true for seeds at the 'table-ready' stage and for roots of nine-day-old seedlings. Our data suggest that both IAA and 4- CI-IAA are required to coordinate the vegetative and reproductive growth of pea plants.

Original languageEnglish (US)
Pages (from-to)675-681
Number of pages7
JournalPhytochemistry
Volume46
Issue number4
DOIs
StatePublished - Oct 1997

Bibliographical note

Funding Information:
Acknowledgements-Supported in part by the Min-nesotaA gricultural ExperimentS tation, Publication Number 961210001A.d ditional funding by grantsn o. IBN-9106039-02 (NSF), OGP0138166 (NSERC), DE-AI02-94ER20153 (DOE), and JF202 (U.S.-Croatian ResearchA greement)t o Mark L. Brermer, Jocelyn A. Ozga,J erry D. Cohen,a nd Volker Magnus is gratefully acknowledgedW. e thank Thomas P. Krick (Departmento f Biochemistry,U niversity of Minnesota) for help and advice with the GC-MS work.

Keywords

  • 4-chloroindole-3-acetic acid
  • Fabaceae
  • Free and conjugated auxin
  • Garden pea
  • Hormone analysis
  • IAA
  • Pisum sativum

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