3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

Michael C. Martin, Charlotte Dabat-Blondeau, Miriam Unger, Julia Sedlmair, Dilworth Y. Parkinson, Hans A. Bechtel, Barbara Illman, Jonathan M. Castro, Marco Keiluweit, David Buschke, Brenda Ogle, Michael J. Nasse, Carol J. Hirschmugl

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Abstract

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

Original languageEnglish (US)
Pages (from-to)861-864
Number of pages4
JournalNature Methods
Volume10
Issue number9
DOIs
StatePublished - Sep 1 2013

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Martin, M. C., Dabat-Blondeau, C., Unger, M., Sedlmair, J., Parkinson, D. Y., Bechtel, H. A., Illman, B., Castro, J. M., Keiluweit, M., Buschke, D., Ogle, B., Nasse, M. J., & Hirschmugl, C. J. (2013). 3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography. Nature Methods, 10(9), 861-864. https://doi.org/10.1038/nmeth.2596