3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

Michael C. Martin, Charlotte Dabat-Blondeau, Miriam Unger, Julia Sedlmair, Dilworth Y. Parkinson, Hans A. Bechtel, Barbara Illman, Jonathan M. Castro, Marco Keiluweit, David Buschke, Brenda Ogle, Michael J. Nasse, Carol J. Hirschmugl

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

Original languageEnglish (US)
Pages (from-to)861-864
Number of pages4
JournalNature Methods
Volume10
Issue number9
DOIs
StatePublished - Sep 1 2013

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Synchrotrons
Three-Dimensional Imaging
Fourier Analysis
Fourier transforms
Infrared radiation
Imaging techniques
Infrared absorption
Data acquisition
Chemical analysis

Cite this

Martin, M. C., Dabat-Blondeau, C., Unger, M., Sedlmair, J., Parkinson, D. Y., Bechtel, H. A., ... Hirschmugl, C. J. (2013). 3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography. Nature Methods, 10(9), 861-864. https://doi.org/10.1038/nmeth.2596

3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography. / Martin, Michael C.; Dabat-Blondeau, Charlotte; Unger, Miriam; Sedlmair, Julia; Parkinson, Dilworth Y.; Bechtel, Hans A.; Illman, Barbara; Castro, Jonathan M.; Keiluweit, Marco; Buschke, David; Ogle, Brenda; Nasse, Michael J.; Hirschmugl, Carol J.

In: Nature Methods, Vol. 10, No. 9, 01.09.2013, p. 861-864.

Research output: Contribution to journalArticle

Martin, MC, Dabat-Blondeau, C, Unger, M, Sedlmair, J, Parkinson, DY, Bechtel, HA, Illman, B, Castro, JM, Keiluweit, M, Buschke, D, Ogle, B, Nasse, MJ & Hirschmugl, CJ 2013, '3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography', Nature Methods, vol. 10, no. 9, pp. 861-864. https://doi.org/10.1038/nmeth.2596
Martin MC, Dabat-Blondeau C, Unger M, Sedlmair J, Parkinson DY, Bechtel HA et al. 3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography. Nature Methods. 2013 Sep 1;10(9):861-864. https://doi.org/10.1038/nmeth.2596
Martin, Michael C. ; Dabat-Blondeau, Charlotte ; Unger, Miriam ; Sedlmair, Julia ; Parkinson, Dilworth Y. ; Bechtel, Hans A. ; Illman, Barbara ; Castro, Jonathan M. ; Keiluweit, Marco ; Buschke, David ; Ogle, Brenda ; Nasse, Michael J. ; Hirschmugl, Carol J. / 3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography. In: Nature Methods. 2013 ; Vol. 10, No. 9. pp. 861-864.
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