(3,2)D GFT-NMR experiments for fast data collection from proteins

Youlin Xia, Guang Zhu, Sudha Veeraraghavan, Xiaolian Gao

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

High throughput structure determination of proteins will contribute to the success of proteomics investigations. The G-Matrix Fourier Transformation NMR (GFT-NMR) method significantly shortens experimental time by reducing the number of the dimensions of data acquisition for isotopically labeled proteins (Kim, S. and Szyperski, T.(2003) J. Am. Chem. Soc. 125, 1385). We demonstrate herein a suite of ten 3D\to2D or (3,2)D GFT-NMR experiments using 13C/15N-labeled ubiquitin. These experiments were completed within 18 hours, representing a 4- to 18-fold reduction in data acquisition time compared to the corresponding conventional 3D experiments. A subset of the GFT-NMR experiments, (3,2)D HNCO, HNCACB, HN(CO)CACB, and 2D 1H-15N HSQC, which are necessary for backbone assignments, were carried out within 6 hours. To facilitate the analysis of the GFT-NMR spectra, we developed automated procedures for viewing and analyzing the GFT-NMR spectra. Our overall strategy allows (3,2)D GFT-NMR experiments to be readily performed and analyzed. Nevertheless, the increase in spectral overlap and the reduction in signal sensitivity in these fast NMR experiments presently limit their application to relatively small proteins.

Original languageEnglish (US)
Pages (from-to)467-476
Number of pages10
JournalJournal of biomolecular NMR
Volume29
Issue number4
DOIs
StatePublished - Aug 2004

Keywords

  • GFT-NMR
  • Multidimensional NMR experiments
  • Protein structure determination
  • RD-NMR

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