2D photonic crystal protein hydrogel coulometer for sensing serum albumin ligand binding

Zhongyu Cai, Jian Tao Zhang, Fei Xue, Zhenmin Hong, David Punihaole, Sanford A. Asher

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Bovine and human serum albumin (BSA and HSA) are globular proteins that function as bloodstream carriers of hydrophobes such as fatty acids and drugs. We fabricated novel photonic crystal protein hydrogels by attaching 2D colloidal arrays onto pure BSA and HSA hydrogels. The wavelengths of the diffracted light sensitively report on the protein hydrogel surface area. The binding of charged species to the protein hydrogel gives rise to Donnan potentials that change the hydrogel volume causing shifts in the diffraction. These photonic crystal protein hydrogels act as sensitive Coulometers that monitor the hydrogel charge state. We find multiple high-affinity BSA and HSA binding sites for salicylate, ibuprofen and picosulfate by using these sensors to monitor binding of charged drugs. We demonstrate proof-of-concept for utilizing protein hydrogel sensors to monitor protein-ionic species binding.

Original languageEnglish (US)
Pages (from-to)4840-4847
Number of pages8
JournalAnalytical chemistry
Volume86
Issue number10
DOIs
StatePublished - May 20 2014

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    Cai, Z., Zhang, J. T., Xue, F., Hong, Z., Punihaole, D., & Asher, S. A. (2014). 2D photonic crystal protein hydrogel coulometer for sensing serum albumin ligand binding. Analytical chemistry, 86(10), 4840-4847. https://doi.org/10.1021/ac404134t