2-(3-Methoxyphenyl)-6, 7-methylenedioxoquinolin-4-one, a novel synthetic compound, inhibited migration and invasion in TSGH8301 human bladder cancer cells

Kuang Chi Lai, Shu Chun Hsu, Jai Sing Yang, Chao Lin Kuo, Siu Wan Ip, Tung Yuan Lai, Shuw Yuan Lin, Ching Che Huang, Sheng Chu Kuo, W. Gibson Wood, Jing Gung Chung

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Matrix metalloproteinases (MMPs) play an important role in the invasion, metastasis and angiogenesis of cancer cells. Many agents have been shown to inhibit the cancer cell migration and invasion by suppression of MMPs. 2-(3-Methoxyphenyl)-6,7-methylenedioxoquinolin-4-one (MMEQ) is a derivative compound synthesized from quinolin and the purpose of this study is to determine whether or not cell migration would be reduced in human bladder cancer TSGH8301 cells after MMEQ treatment. Wound healing assay and boyden chamber assay were used in cell migration and invasion determinations. Cell migration and invasion inhibited by MMEQ exerted an inhibitory effect on the sevenless homolog-1 (SOS-1), protein kinase c (PKC), extracellular signal-regulated kinase (ERK) and Rho A for causing the inhibitions of MMP-2 and -9, and then followed by the inhibitions of invasion and migration. MMEQ also affected FAK, PI3K or inhibited growth factor receptor-bound protein 2 (GRB2), nuclear factor kappaB (NF-κB), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) for cell proliferation inhibition. Therefore, MMEQ may serve as a drug in the prevention of tumor metastasis of bladder cancer in the future.

Original languageEnglish (US)
Pages (from-to)1045-1052
Number of pages8
JournalHuman and Experimental Toxicology
Volume30
Issue number8
DOIs
StatePublished - Aug 1 2011

Keywords

  • 2-(3-methoxyphenyl)-6
  • 7-methylenedioxoquinolin-4-one (MMEQ)
  • TSGH8301 human bladder cancer cells
  • cell invasion
  • cell migration

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    Lai, K. C., Hsu, S. C., Yang, J. S., Kuo, C. L., Ip, S. W., Lai, T. Y., Lin, S. Y., Huang, C. C., Kuo, S. C., Gibson Wood, W., & Chung, J. G. (2011). 2-(3-Methoxyphenyl)-6, 7-methylenedioxoquinolin-4-one, a novel synthetic compound, inhibited migration and invasion in TSGH8301 human bladder cancer cells. Human and Experimental Toxicology, 30(8), 1045-1052. https://doi.org/10.1177/0960327110386257