甲烷丝菌混养促进螺旋藻自发酵产氢

The effect of Methanothrix concilii, which consumes acetate as its lone energy source, on hydrogen production from Arthrospira (Spirulina) maxima through autofermentation was investigated for the first time. During A. maxima cultivation, the effect of different NO ₃ ^- and Na ⁺ concentrations on cell growth and the accumulation of carbohydrates was also explored. At a Na ⁺ concentration of 739 mmol•L ^-1 , the total carbohydrate yield increased by 107.7% to 0.54 g•L ^-1 . The harvested A. maxima suspension was treated under dark and anaerobic conditions to produce hydrogen in autofermentation, with M. concilii added during the fermentation. By adding M. concilii, the hydrogen yield reached up to 43.8 mL•g ^-1 , an increase of 33.8%. Furthermore, the acetate, as the predominant soluble metabolite product, increased by 69.2% to 1639.1 μmol•g ^-1 , whilst the energy conversion efficiency was enhanced from 6.7% to 11.6%. By consuming acetate, M. concilii facilitated the acetate metabolic pathway in the autofermentation of A. maxima, resulting in the enhanced hydrogen yield and significantly increased energy conversion efficiency.