β-Actin specifically controls cell growth, migration, and the G-actin pool

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Ubiquitously expressed β-actin and γ-actin isoforms play critical roles in most cellular processes; however, their unique contributions are not well understood. We generated whole-body β-actin-knockout (Actb -/-) mice and demonstrated that β-actin is required for early embryonic development. Lethality of Actb -/- embryos correlated with severe growth impairment and migration defects in β-actin-knockout primary mouse embryonic fibroblasts (MEFs) that were not observed in γ-actin-null MEFs. Migration defects were associated with reduced membrane protrusion dynamics and increased focal adhesions. We also identified migration defects upon conditional ablation of β-actin in highly motile T cells. Of great interest, ablation of β-actin altered the ratio of globular actin (G-actin) to filamentous actin in MEFs, with corresponding changes in expression of genes that regulate the cell cycle and motility. These data support an essential role for β-actin in regulating cell migration and gene expression through control of the cellular G-actin pool.

Original languageEnglish (US)
Pages (from-to)4047-4058
Number of pages12
JournalMolecular biology of the cell
Volume22
Issue number21
DOIs
StatePublished - Nov 1 2011

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Cell Movement
Actins
Growth
Fibroblasts
Knockout Mice
Gene Expression
Focal Adhesions
Embryonic Development
Protein Isoforms
Embryonic Structures
T-Lymphocytes
Membranes

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β-Actin specifically controls cell growth, migration, and the G-actin pool. / Bunnell, Tina M.; Burbach, Brandon J; Shimizu, Yoji; Ervasti, James M.

In: Molecular biology of the cell, Vol. 22, No. 21, 01.11.2011, p. 4047-4058.

Research output: Contribution to journalArticle

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