α-Hydroxylation of N-nitrosopyrrolidine and N′-nitrosonornicotine by human liver microsomes

Stephen S. Hecht; Chi Hong B. Chen; G. David McCoy; Dietrich Hoffmann; Lennart Domellöf

doi:10.1016/0304-3835(79)90020-X

α-Hydroxylation of N-nitrosopyrrolidine and N′-nitrosonornicotine by human liver microsomes

Stephen S. Hecht, Chi Hong B. Chen, G. David McCoy, Dietrich Hoffmann, Lennart Domellöf

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The environmental carcinogens N-nitrosopyrrolidine (NPYR) and N′-nitrosonornicotine (NNN) were incubated with human liver microsomes to test for the presence of enzymes catalyzing α-hydroxylation, which is likely to be an activation mechanism for these compounds. Both nitrosamines underwent α-hydroxylation; rates were higher for NPYR than for NNN, as observed in rats. The results indicate that both NPYR and NNN can be metabolically activated by human liver microsomes.

Original language	English (US)
Pages (from-to)	35-41
Number of pages	7
Journal	Cancer Letters
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(79)90020-X
State	Published - Nov 1979

Bibliographical note

Funding Information:
This study was supported by National Cancer Institute Grants CA 21393 and CA 23901. Stephen S. Hecht is recipient of National Cancer Institute Research Career Development Award 5K04-CA 00124.

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10.1016/0304-3835(79)90020-X

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title = "α-Hydroxylation of N-nitrosopyrrolidine and N′-nitrosonornicotine by human liver microsomes",

abstract = "The environmental carcinogens N-nitrosopyrrolidine (NPYR) and N′-nitrosonornicotine (NNN) were incubated with human liver microsomes to test for the presence of enzymes catalyzing α-hydroxylation, which is likely to be an activation mechanism for these compounds. Both nitrosamines underwent α-hydroxylation; rates were higher for NPYR than for NNN, as observed in rats. The results indicate that both NPYR and NNN can be metabolically activated by human liver microsomes.",

author = "Hecht, {Stephen S.} and Chen, {Chi Hong B.} and McCoy, {G. David} and Dietrich Hoffmann and Lennart Domell{\"o}f",

note = "Funding Information: This study was supported by National Cancer Institute Grants CA 21393 and CA 23901. Stephen S. Hecht is recipient of National Cancer Institute Research Career Development Award 5K04-CA 00124.",

year = "1979",

month = nov,

doi = "10.1016/0304-3835(79)90020-X",

language = "English (US)",

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T1 - α-Hydroxylation of N-nitrosopyrrolidine and N′-nitrosonornicotine by human liver microsomes

AU - Hecht, Stephen S.

AU - Chen, Chi Hong B.

AU - McCoy, G. David

AU - Hoffmann, Dietrich

AU - Domellöf, Lennart

N1 - Funding Information: This study was supported by National Cancer Institute Grants CA 21393 and CA 23901. Stephen S. Hecht is recipient of National Cancer Institute Research Career Development Award 5K04-CA 00124.

PY - 1979/11

Y1 - 1979/11

N2 - The environmental carcinogens N-nitrosopyrrolidine (NPYR) and N′-nitrosonornicotine (NNN) were incubated with human liver microsomes to test for the presence of enzymes catalyzing α-hydroxylation, which is likely to be an activation mechanism for these compounds. Both nitrosamines underwent α-hydroxylation; rates were higher for NPYR than for NNN, as observed in rats. The results indicate that both NPYR and NNN can be metabolically activated by human liver microsomes.

AB - The environmental carcinogens N-nitrosopyrrolidine (NPYR) and N′-nitrosonornicotine (NNN) were incubated with human liver microsomes to test for the presence of enzymes catalyzing α-hydroxylation, which is likely to be an activation mechanism for these compounds. Both nitrosamines underwent α-hydroxylation; rates were higher for NPYR than for NNN, as observed in rats. The results indicate that both NPYR and NNN can be metabolically activated by human liver microsomes.

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α-Hydroxylation of N-nitrosopyrrolidine and N′-nitrosonornicotine by human liver microsomes

Abstract

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