The dystrophin-glycoprotein complex was examined in dystrophin-deficient dogs with golden retriever muscular dystrophy (GRMD) using immunoblot and immunofluorescence analysis. The dystrophin-associated proteins were substantially reduced in muscle from dogs with GRMD. Interestingly, regression analysis revealed a strong correlation between the amount of α-dystroglycan and serum creatine kinase levels and the contraction tension measured for a given peroneus longus muscle.
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Acknowledgements: We gratefully acknowledgeD avid Zoloto and Michael Mullinix for preparationo f the skeletaml uscleh omogenates and membranesu sed in this study. K.P.C. is an investigatoro f the Howard HughesM edical Institute.T his work was also supportedb y grants from the Muscular Dystrophy Association (J.M.E., S.L.R., J.N.K. and K.P.C.) and the WisconsinA ffiliate of the AmericanH eart Association( J.M.E.).
- Dystrophin-associated protein
- Dystrophin-glycoprotein complex
- Golden retriever muscular dystrophy