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Research Output 1981 2013

2013
biology
2012
3 Citations (Scopus)

A facile transport assay for H + coupled membrane transport using fluorescence probes

Lan, W., Ren, H., Pang, Y., Huang, C., Xu, Y., Brooker, R. J. & Zhang, J., Jan 1 2012, In : Analytical Methods. 4, 1, p. 44-46 3 p.

Research output: Contribution to journalArticle

Assays
Fluorescence
Membranes
Protons
Manganese
2010
12 Citations (Scopus)

Evidence that highly conserved residues of transmembrane segment 6 of escherichia coli mnth are important for transport activity

Haemig, H. A. H., Moen, P. J. & Brooker, R. J., Jun 8 2010, In : Biochemistry. 49, 22, p. 4662-4671 10 p.

Research output: Contribution to journalArticle

Escherichia coli
Metals
Histidine
Symporters
Mutagenesis
2006
3 Citations (Scopus)
Cations
Melibiose
Galactosides
Lactose
Amino Acids
2004
6 Citations (Scopus)

Control of H+/lactose coupling by ionic interactions in the lactose permease of escherichia coli

Johnson, J. L. & Brooker, R. J., Apr 1 2004, In : Journal of Membrane Biology. 198, 3, p. 135-146 12 p.

Research output: Contribution to journalArticle

Lactose
Escherichia coli
Protons
Melibiose
Membrane Transport Proteins
33 Citations (Scopus)
Site-Directed Mutagenesis
Computational Biology
Cytoplasm
Metals
Escherichia coli
2003
9 Citations (Scopus)
Lactose
Escherichia coli
Mutation
Biochemistry
Membrane Transport Proteins
3 Citations (Scopus)

Role of glutamate-126 and arginine-144 in the lactose permease of Escherichia coli

Johnson, J. L. & Brooker, R. J., Feb 4 2003, In : Biochemistry. 42, 4, p. 1095-1100 6 p.

Research output: Contribution to journalArticle

arginine glutamate
Lactose
Escherichia coli
Arginine
Glutamic Acid
2002
4 Citations (Scopus)

An isotope-edited FT-IR study of a symporter, the lactose permease

Patzlaff, J. S., Zhang, J., Brooker, R. J. & Barry, B. A., Jun 11 2002, In : Biochemistry. 41, 23, p. 7366-7372 7 p.

Research output: Contribution to journalArticle

Symporters
Membrane Transport Proteins
Isotopes
Carboxylic Acids
Lactose
2001
5 Citations (Scopus)

A face on transmembrane segment 8 of the lactose permease is important for transport activity

Green, A. L. & Brooker, R. J., Oct 9 2001, In : Biochemistry. 40, 40, p. 12220-12229 10 p.

Research output: Contribution to journalArticle

Lactose
Substitution reactions
Amino Acids
Mutagenesis
Active Biological Transport
12 Citations (Scopus)

A triple mutant, K319N/H322Q/E325Q, of the lactose permease cotransports H+ with thiodigalactoside

Johnson, J. L., Lockheart, M. S. K. & Brooker, R. J., Jun 1 2001, In : Journal of Membrane Biology. 181, 3, p. 215-224 10 p.

Research output: Contribution to journalArticle

Lactose
Genetic Suppression
Ion Transport
Codon
Cations
2000
3 Citations (Scopus)

A reaction-induced fourier transform-infrared spectroscopic study of the lactose permease. A transmembrane potential perturbs carboxylic acid residues

Patzlaff, J. S., Brooker, R. J. & Barry, B. A., Sep 15 2000, In : Journal of Biological Chemistry. 275, 37, p. 28695-28700 6 p.

Research output: Contribution to journalArticle

Membrane Transport Proteins
Fourier Analysis
Carboxylic Acids
Membrane Potentials
Fourier transforms
18 Citations (Scopus)
Lactose
Substitution reactions
Codon
Glycine
Mutagenesis
15 Citations (Scopus)

Roles of charged residues in the conserved motif, G-X-X-X-D/E-R/K-X-G- [X]-R/K-R/K, of the lactose permease of Escherichia coli

Pazdernik, N. J., Matzke, E. A., Jessen-Marshall, A. E. & Brooker, R. J., Mar 15 2000, In : Journal of Membrane Biology. 174, 1, p. 31-40 10 p.

Research output: Contribution to journalArticle

Genetic Suppression
Protein Stability
Lactose
Aspartic Acid
Membrane Proteins
15 Citations (Scopus)

The conserved motif in hydrophilic loop 2/3 and loop 8/9 of the lactose permease of Escherichia coli. Analysis of suppressor mutations

Cain, S. M., Matzke, E. A. & Brooker, R. J., Aug 19 2000, In : Journal of Membrane Biology. 176, 2, p. 159-168 10 p.

Research output: Contribution to journalArticle

Genetic Suppression
Escherichia coli
Mutation
Lactose
Membrane Transport Proteins
1999
18 Citations (Scopus)
Ion Transport
Lactose
Alanine
Active Biological Transport
Sugars
1998
60 Citations (Scopus)

Fourier transform infrared analysis of purified lactose permease: A monodisperse lactose permease preparation is stably folded, α-helical, and highly accessible to deuterium exchange

Patzlaff, J. S., Moeller, J. A., Barry, B. A. & Brooker, R. J., Nov 3 1998, In : Biochemistry. 37, 44, p. 15363-15375 13 p.

Research output: Contribution to journalArticle

Deuterium
Fourier Analysis
Fourier transforms
Infrared radiation
Fourier Transform Infrared Spectroscopy
1997
20 Citations (Scopus)
Genetic Suppression
Lactose
Mutation
Substitution reactions
Amino Acid Substitution
17 Citations (Scopus)
Melibiose
Lactose
Escherichia coli
Galactosides
Nitrophenylgalactosides
17 Citations (Scopus)

Role of conserved residues in hydrophilic loop 8-9 of the lactose permease

Pazdernik, N. J., Jessen-Marshall, A. E. & Brooker, R. J., Jan 1 1997, In : Journal of Bacteriology. 179, 3, p. 735-741 7 p.

Research output: Contribution to journalArticle

Lactose
Membrane Transport Proteins
Glycine
Asparagine
Amino Acid Substitution
31 Citations (Scopus)
Genetic Suppression
Lactose
Glycine
Membrane Transport Proteins
Valine
1996
43 Citations (Scopus)
Aspartic Acid
Genetic Suppression
Mutation
Topology
Proteins
6 Citations (Scopus)

Structural topology of transmembrane helix 10 in the lactose permease of Escherichia coli

Goswitz, V. C., Matzke, E. A., Taylor, M. R., Jessen-Marshall, A. E. & Brooker, R. J., Sep 25 1996, In : Journal of Biological Chemistry. 271, 36, p. 21927-21932 6 p.

Research output: Contribution to journalArticle

Escherichia coli
Topology
Amino Acids
Substitution reactions
Amino Acid Substitution
1995
79 Citations (Scopus)

Structural features of the uniporter/symporter/antiporter superfamily

Goswitz, V. C. & Brooker, R. J., Jan 1 1995, In : Protein Science. 4, 3, p. 534-537 4 p.

Research output: Contribution to journalArticle

Antiporters
Symporters
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
76 Citations (Scopus)
Glycine
Substitution reactions
Mutation
Membrane Transport Proteins
Active Biological Transport
1994
76 Citations (Scopus)

Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli

Franco, P. J. & Brooker, R. J., Mar 11 1994, In : Journal of Biological Chemistry. 269, 10, p. 7379-7386 8 p.

Research output: Contribution to journalArticle

Lactose
Escherichia coli
Sugars
Galactosides
Mutation
1993
19 Citations (Scopus)

Isolation of lactose permease mutants which recognize arabinose

Goswitz, V. C. & Brooker, R. J., Jan 1 1993, In : Molecular Membrane Biology. 10, 1, p. 61-70 10 p.

Research output: Contribution to journalArticle

Arabinose
Sugars
Monosaccharides
Lactose
Membrane Transport Proteins
20 Citations (Scopus)

Lactose permease mutants which transport (malto-)oligosaccharides

Olsen, S. G., Greene, K. M. & Brooker, R. J., Jan 1 1993, In : Journal of Bacteriology. 175, 19, p. 6269-6275 7 p.

Research output: Contribution to journalArticle

Oligosaccharides
Trisaccharides
Membrane Transport Proteins
Amino Acid Substitution
Fermentation
1992
7 Citations (Scopus)
Maltose
Membrane Transport Proteins
Isoleucine
Valine
Lactose
27 Citations (Scopus)

Functional role of arginine 302 within the lactose permease of Escherichia coli

Matzke, E. A., Stephenson, L. J. & Brooker, R. J., Sep 25 1992, In : Journal of Biological Chemistry. 267, 27, p. 19095-19100 6 p.

Research output: Contribution to journalArticle

Lactose
Escherichia coli
Arginine
Assays
Sugars
1991
17 Citations (Scopus)
Membrane Transport Proteins
Lactose
Sugars
Protons
Galactosides
30 Citations (Scopus)
Galactosides
Membrane Transport Proteins
Ion Transport
Lactose
Sugars
13 Citations (Scopus)
Galactosides
Asparagine
Lactose
Stoichiometry
Protons
1990
23 Citations (Scopus)

Characterization of the double mutant, Val-177/Asn-322, of the lactose permease

Brooker, R. J., Mar 5 1990, In : Journal of Biological Chemistry. 265, 7, p. 4155-4160 6 p.

Research output: Contribution to journalArticle

Lactose
Thiogalactosides
Sugars
Galactosides
Defects
20 Citations (Scopus)
Protein Conformation
Molecular Models
Membrane Transport Proteins
Active Biological Transport
Substrate Specificity
1989
29 Citations (Scopus)
Galactosides
Sugars
Lactose
Benzene
Hexoses
10 Citations (Scopus)

Characterization and sequencing of the lac Y54-41 'uncoupled' mutant of the lactose permease

Brooker, R. J., Myster, S. H. & Wilson, T. H., Jan 1 1989, In : Journal of Biological Chemistry. 264, 14, p. 8135-8140 6 p.

Research output: Contribution to journalArticle

Membrane Transport Proteins
Genes
Thiogalactosides
Sulfhydryl Reagents
Lactose
48 Citations (Scopus)

Isolation and characterization of lactose permease mutants with an enhanced recognition of maltose and diminished recognition of cellobiose.

Collins, J. C., Permuth, S. F. & Brooker, R. J., Jan 1 1989, In : The Journal of biological chemistry. 264, 25, p. 14698-14703 6 p.

Research output: Contribution to journalArticle

Cellobiose
Maltose
Galactosides
Substitution reactions
Membrane Transport Proteins
18 Citations (Scopus)

Isolation and characterization of thiodigalactoside-resistant mutants of the lactose permease which possess an enhanced recognition for maltose

Franco, P. J., Eelkema, J. A. & Brooker, R. J., Jan 1 1989, In : Journal of Biological Chemistry. 264, 27, p. 15988-15992 5 p.

Research output: Contribution to journalArticle

Maltose
Substitution reactions
Asparagine
Amino Acid Substitution
Lactose
1987
40 Citations (Scopus)
Membrane Transport Proteins
Escherichia coli
Cysteine
Ion Transport
Lactose
1986
21 Citations (Scopus)
Galactosides
Lactose
Escherichia coli
Cysteine
Sulfhydryl Reagents
1985
14 Citations (Scopus)

Characterization of lactose carrier mutants which transport maltose

Brooker, R. J., Fiebig, K. & Wilson, T. H., Dec 1 1985, In : Journal of Biological Chemistry. 260, 30, p. 16181-16186 6 p.

Research output: Contribution to journalArticle

Methylgalactosides
Thiogalactosides
Species Specificity
Maltose
Membrane Transport Proteins
2 Citations (Scopus)
Lipid bilayers
Maltose
Membrane Transport Proteins
Active Biological Transport
Lipid Bilayers
56 Citations (Scopus)
Maltose
Lactose
Nucleotides
Galactosides
Alanine
1984
73 Citations (Scopus)

Electrogenic H+ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme

Perlin, D. S., Kasamo, K., Brooker, R. J. & Slayman, C. W., Jan 1 1984, In : Journal of Biological Chemistry. 259, 12, p. 7884-7892 9 p.

Research output: Contribution to journalArticle

Neurospora
Cell membranes
Adenosine Triphosphatases
Hydrolysis
Acridine Orange
1 Citation (Scopus)
DNA Sequence Analysis
Organism Cloning
1983
29 Citations (Scopus)
Neurospora crassa
Ethylmaleimide
Proton-Translocating ATPases
Cell membranes
Labeling
20 Citations (Scopus)

Effects of Mg2+ ions on the plasma membrane [H+]-ATPase of Neurospora crassa. I. Inhibition by N-ethylmaleimide and trypsin.

Brooker, R. J. & Slayman, C. W., Jul 25 1983, In : Journal of Biological Chemistry. 258, 14, p. 8827-8832 6 p.

Research output: Contribution to journalArticle

Neurospora crassa
Ethylmaleimide
Proton-Translocating ATPases
Cell membranes
Trypsin
27 Citations (Scopus)
Neurospora crassa
Proton-Translocating ATPases
Cell membranes
Adenosine Triphosphate
Cell Membrane
1982
36 Citations (Scopus)
Neurospora
Neurospora crassa
Ethylmaleimide
Proton-Translocating ATPases
Cell membranes