Robert J Brooker – Research output — Experts@Minnesota

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Emailbrook005umnedu

Research activity per year

50 Article
1 Chapter

1 - 50 out of 51 results

2013

Scale-up in a large introductory biology course
Brooker, R., Matthes, D., Wright, R., Wassenberg, D., Wick, S. & Couch, B., Jan 1 2013, Connected Science: Strategies for Integrative Learning in College. Indiana University Press, p. 89-99 11 p.
Research output: Chapter in Book/Report/Conference proceeding › Chapter
2012
A facile transport assay for H ⁺ coupled membrane transport using fluorescence probes
Lan, W., Ren, H., Pang, Y., Huang, C., Xu, Y., Brooker, R. J. & Zhang, J., Jan 2012, In: Analytical Methods. 4, 1, p. 44-46 3 p.
Research output: Contribution to journal › Article › peer-review
5 Scopus citations
2010
Evidence that highly conserved residues of transmembrane segment 6 of escherichia coli mnth are important for transport activity
Haemig, H. A. H., Moen, P. J. & Brooker, R. J., Jun 8 2010, In: Biochemistry. 49, 22, p. 4662-4671 10 p.
Research output: Contribution to journal › Article › peer-review

Open Access
18 Scopus citations
2006
A suppressor analysis of residues involved in cation transport in the lactose permease: Identification of a coupling sensor
Franco, P. J., Matzke, E. A., Johnson, J. L., Wiczer, B. M. & Brooker, R. J., May 1 2006, In: Journal of Membrane Biology. 211, 2, p. 101-113 13 p.
Research output: Contribution to journal › Article › peer-review
3 Scopus citations
2004
Control of H⁺/lactose coupling by ionic interactions in the lactose permease of escherichia coli
Johnson, J. L. & Brooker, R. J., Apr 1 2004, In: Journal of Membrane Biology. 198, 3, p. 135-146 12 p.
Research output: Contribution to journal › Article › peer-review
6 Scopus citations
Importance of conserved acidic residues in MntH, the Nramp homolog of Escherichia coli
Haemig, H. A. H. & Brooker, R. J., Nov 2004, In: Journal of Membrane Biology. 201, 2, p. 97-107 11 p.
Research output: Contribution to journal › Article › peer-review
40 Scopus citations
2003
Evidence for structural symmetry and functional asymmetry in the lactose permease of Escherichia coli
Green, A. L., Hrodey, H. A. & Brooker, R. J., Sep 30 2003, In: Biochemistry. 42, 38, p. 11226-11233 8 p.
Research output: Contribution to journal › Article › peer-review
9 Scopus citations
Role of glutamate-126 and arginine-144 in the lactose permease of Escherichia coli
Johnson, J. L. & Brooker, R. J., Feb 4 2003, In: Biochemistry. 42, 4, p. 1095-1100 6 p.
Research output: Contribution to journal › Article › peer-review
3 Scopus citations
2002
An isotope-edited FT-IR study of a symporter, the lactose permease
Patzlaff, J. S., Zhang, J., Brooker, R. J. & Barry, B. A., Jun 11 2002, In: Biochemistry. 41, 23, p. 7366-7372 7 p.
Research output: Contribution to journal › Article › peer-review
5 Scopus citations
2001
A face on transmembrane segment 8 of the lactose permease is important for transport activity
Green, A. L. & Brooker, R. J., Oct 9 2001, In: Biochemistry. 40, 40, p. 12220-12229 10 p.
Research output: Contribution to journal › Article › peer-review
5 Scopus citations
A triple mutant, K319N/H322Q/E325Q, of the lactose permease cotransports H⁺ with thiodigalactoside
Johnson, J. L., Lockheart, M. S. K. & Brooker, R. J., Jun 1 2001, In: Journal of Membrane Biology. 181, 3, p. 215-224 10 p.
Research output: Contribution to journal › Article › peer-review
13 Scopus citations
2000
A reaction-induced fourier transform-infrared spectroscopic study of the lactose permease. A transmembrane potential perturbs carboxylic acid residues
Patzlaff, J. S., Brooker, R. J. & Barry, B. A., Sep 15 2000, In: Journal of Biological Chemistry. 275, 37, p. 28695-28700 6 p.
Research output: Contribution to journal › Article › peer-review

Open Access
3 Scopus citations
A revised model for the structure and function of the lactose permease: Evidence that a face on transmembrane segment 2 is important for conformational changes
Green, A. L., Anderson, E. J. & Brooker, R. J., Jul 28 2000, In: Journal of Biological Chemistry. 275, 30, p. 23240-23246 7 p.
Research output: Contribution to journal › Article › peer-review
18 Scopus citations
Roles of charged residues in the conserved motif, G-X-X-X-D/E-R/K-X-G- [X]-R/K-R/K, of the lactose permease of Escherichia coli
Pazdernik, N. J., Matzke, E. A., Jessen-Marshall, A. E. & Brooker, R. J., 2000, In: Journal of Membrane Biology. 174, 1, p. 31-40 10 p.
Research output: Contribution to journal › Article › peer-review
15 Scopus citations
The conserved motif in hydrophilic loop 2/3 and loop 8/9 of the lactose permease of Escherichia coli. Analysis of suppressor mutations
Cain, S. M., Matzke, E. A. & Brooker, R. J., Aug 19 2000, In: Journal of Membrane Biology. 176, 2, p. 159-168 10 p.
Research output: Contribution to journal › Article › peer-review
17 Scopus citations
1999
A K319N/E325Q double mutant of the lactose permease cotransports H⁺ with lactose: Implications for a proposed mechanism of H⁺/lactose symport
Johnson, J. L. & Brooker, R. J., Feb 12 1999, In: Journal of Biological Chemistry. 274, 7, p. 4074-4081 8 p.
Research output: Contribution to journal › Article › peer-review
18 Scopus citations
1998
Fourier transform infrared analysis of purified lactose permease: A monodisperse lactose permease preparation is stably folded, α-helical, and highly accessible to deuterium exchange
Patzlaff, J. S., Moeller, J. A., Barry, B. A. & Brooker, R. J., Nov 3 1998, In: Biochemistry. 37, 44, p. 15363-15375 13 p.
Research output: Contribution to journal › Article › peer-review
61 Scopus citations
1997
An analysis of suppressor mutations suggests that the two halves of the lactose permease function in a symmetrical manner
Pazdernik, N. J., Cain, S. M. & Brooker, R. J., Oct 17 1997, In: Journal of Biological Chemistry. 272, 42, p. 26110-26116 7 p.
Research output: Contribution to journal › Article › peer-review
21 Scopus citations
Lactose carrier mutants of Escherichia coli with changes in sugar recognition (Lactose versus melibiose)
Varela, M. F., Brooker, R. J. & Wilson, T. H., Sep 1997, In: Journal of bacteriology. 179, 17, p. 5570-5573 4 p.
Research output: Contribution to journal › Article › peer-review

Open Access
18 Scopus citations
Role of conserved residues in hydrophilic loop 8-9 of the lactose permease
Pazdernik, N. J., Jessen-Marshall, A. E. & Brooker, R. J., 1997, In: Journal of bacteriology. 179, 3, p. 735-741 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
17 Scopus citations
Suppressor analysis of mutations in the loop 2-3 motif of lactose permease: Evidence that glycine-64 is an important residue for conformational changes
Jessen-Marshall, A. E., Parker, N. J. & Brooker, R. J., 1997, In: Journal of bacteriology. 179, 8, p. 2616-2622 7 p.
Research output: Contribution to journal › Article › peer-review
33 Scopus citations
1996
Evidence that transmembrane segment 2 of the lactose permease is part of a conformationally sensitive interface between the two halves of the protein
Jessen-Marshall, A. E. & Brooker, R. J., Jan 19 1996, In: Journal of Biological Chemistry. 271, 3, p. 1400-1404 5 p.
Research output: Contribution to journal › Article › peer-review
43 Scopus citations
Structural topology of transmembrane helix 10 in the lactose permease of Escherichia coli
Goswitz, V. C., Matzke, E. A., Taylor, M. R., Jessen-Marshall, A. E. & Brooker, R. J., 1996, In: Journal of Biological Chemistry. 271, 36, p. 21927-21932 6 p.
Research output: Contribution to journal › Article › peer-review
6 Scopus citations
1995
Structural features of the uniporter/symporter/antiporter superfamily
Goswitz, V. C. & Brooker, R. J., Mar 1995, In: Protein Science. 4, 3, p. 534-537 4 p.
Research output: Contribution to journal › Article › peer-review

Open Access
85 Scopus citations
The conserved motif, GXXX(D/E)(R/K)XG[X](R/K)(R/K), in hydrophilic loop 2/3 of the lactose permease
Jessen-Marshall, A. E., Paul, N. J. & Brooker, R. J., Jul 7 1995, In: Journal of Biological Chemistry. 270, 27, p. 16251-16257 7 p.
Research output: Contribution to journal › Article › peer-review
83 Scopus citations
1994
Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli
Franco, P. J. & Brooker, R. J., Mar 11 1994, In: Journal of Biological Chemistry. 269, 10, p. 7379-7386 8 p.
Research output: Contribution to journal › Article › peer-review
78 Scopus citations
1993
Isolation of lactose permease mutants which recognize arabinose
Goswitz, V. C. & Brooker, R. J., 1993, In: Molecular Membrane Biology. 10, 1, p. 61-70 10 p.
Research output: Contribution to journal › Article › peer-review
20 Scopus citations
Lactose permease mutants which transport (malto-)oligosaccharides
Olsen, S. G., Greene, K. M. & Brooker, R. J., 1993, In: Journal of bacteriology. 175, 19, p. 6269-6275 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
21 Scopus citations
1992
An analysis of the side chain requirement at position 177 within the lactose permease which confers the ability to recognize maltose
Gram, C. D. & Brooker, R. J., Feb 25 1992, In: Journal of Biological Chemistry. 267, 6, p. 3841-3846 6 p.
Research output: Contribution to journal › Article › peer-review
9 Scopus citations
Functional role of arginine 302 within the lactose permease of Escherichia coli
Matzke, E. A., Stephenson, L. J. & Brooker, R. J., Sep 25 1992, In: Journal of Biological Chemistry. 267, 27, p. 19095-19100 6 p.
Research output: Contribution to journal › Article › peer-review
29 Scopus citations
1991
An analysis of lactose permease "sugar specificity" mutations which also affect the coupling between proton and lactose transport I. VAL¹⁷⁷ and VAL¹⁷⁷/ASN³¹⁹ permeases facilitate proton uniport and sugar uniport
Brooker, R. J., Mar 5 1991, In: Journal of Biological Chemistry. 266, 7, p. 4131-4138 8 p.
Research output: Contribution to journal › Article › peer-review
42 Scopus citations
An analysis of lactose permease "sugar specificity" mutations which also affect the coupling between proton and lactose transport II. Second site revertants of the thiodigalactoside-dependent proton leak by the VAL¹⁷⁷/ASN³¹⁹ permease
Eelkema, J. A., O'Donnell, M. A. & Brooker, R. J., Mar 5 1991, In: Journal of Biological Chemistry. 266, 7, p. 4139-4144 6 p.
Research output: Contribution to journal › Article › peer-review
19 Scopus citations
Evidence that the asparagine, 322 mutant of the lactose permease transports protons and lactose with a normal stoichiometry and accumulates lactose against a concentration gradient
Franco, P. J. & Brooker, R. J., 1991, In: Journal of Biological Chemistry. 266, 11, p. 6693-6699 7 p.
Research output: Contribution to journal › Article › peer-review
20 Scopus citations
1990
Characterization of the double mutant, Val-177/Asn-322, of the lactose permease
Brooker, R. J., Mar 5 1990, In: Journal of Biological Chemistry. 265, 7, p. 4155-4160 6 p.
Research output: Contribution to journal › Article › peer-review
30 Scopus citations
The lactose permease of Escherichia coli
Brooker, R. J., 1990, In: Research in Microbiology. 141, 3, p. 309-315 7 p.
Research output: Contribution to journal › Article › peer-review

28 Scopus citations
1989
Analysis of the structural specificity of the lactose permease toward sugars
Olsen, S. G. & Brooker, R. J., 1989, In: Journal of Biological Chemistry. 264, 27, p. 15982-15987 6 p.
Research output: Contribution to journal › Article › peer-review
34 Scopus citations
Characterization and sequencing of the lac Y^54-41 'uncoupled' mutant of the lactose permease
Brooker, R. J., Myster, S. H. & Wilson, T. H., 1989, In: Journal of Biological Chemistry. 264, 14, p. 8135-8140 6 p.
Research output: Contribution to journal › Article › peer-review
17 Scopus citations
Isolation and characterization of lactose permease mutants with an enhanced recognition of maltose and diminished recognition of cellobiose.
Collins, J. C., Permuth, S. F. & Brooker, R. J., Sep 5 1989, In: The Journal of biological chemistry. 264, 25, p. 14698-14703 6 p.
Research output: Contribution to journal › Article › peer-review
63 Scopus citations
Isolation and characterization of thiodigalactoside-resistant mutants of the lactose permease which possess an enhanced recognition for maltose
Franco, P. J., Eelkema, J. A. & Brooker, R. J., 1989, In: Journal of Biological Chemistry. 264, 27, p. 15988-15992 5 p.
Research output: Contribution to journal › Article › peer-review
27 Scopus citations
1987
Role of Cysteine Residues in the Lac Permease of Escherichia coli
Menick, D. R., Lee, J. A., Kaback, H. R., Brooker, R. J. & Wilson, T. H., 1987, In: Biochemistry. 26, 4, p. 1132-1136 5 p.
Research output: Contribution to journal › Article › peer-review
44 Scopus citations
1986
Site-specific alteration of cysteine 176 and cysteine 234 in the lactose carrier of Escherichia coli
Brooker, R. J. & Wilson, T. H., 1986, In: Journal of Biological Chemistry. 261, 25, p. 11765-11769 5 p.
Research output: Contribution to journal › Article › peer-review
24 Scopus citations
1985

Characterization of lactose carrier mutants which transport maltose
Brooker, R. J., Fiebig, K. & Wilson, T. H., 1985, In: Journal of Biological Chemistry. 260, 30, p. 16181-16186 6 p.
Research output: Contribution to journal › Article › peer-review

24 Scopus citations
Isolation, Characterization, and Nucleotide Sequences of Lactose Permease Mutants That Have Acquired the Ability to Transport Maltose
BROOKER, R. J. & WILSON, T. H., Nov 1985, In: Annals of the New York Academy of Sciences. 456, 1, p. 350 1 p.
Research output: Contribution to journal › Article › peer-review

2 Scopus citations
Isolation and nucleotide sequencing of lactose carrier mutants that transport maltose
Brooker, R. J. & Wilson, T. H., 1985, In: Proceedings of the National Academy of Sciences of the United States of America. 82, 12, p. 3959-3963 5 p.
Research output: Contribution to journal › Article › peer-review

Open Access
69 Scopus citations
1984
Electrogenic H⁺ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme
Perlin, D. S., Kasamo, K., Brooker, R. J. & Slayman, C. W., 1984, In: Journal of Biological Chemistry. 259, 12, p. 7884-7892 9 p.
Research output: Contribution to journal › Article › peer-review
80 Scopus citations
Mapping, cloning, and DNA sequencing of lac Y mutants
Brooker, R. J. & Wilson, T. H., Jan 1 1984, In: Federation Proceedings. 43, 6, p. no. 1380
Research output: Contribution to journal › Article › peer-review

1 Scopus citations
1983
[14C]N-ethylmaleimide labeling of the plasma membrane [H+]-ATPase of Neurospora crassa.
Brooker, R. J. & Slayman, C. W., Jan 10 1983, In: Journal of Biological Chemistry. 258, 1, p. 222-226 5 p.
Research output: Contribution to journal › Article › peer-review
31 Scopus citations
Effects of Mg2+ ions on the plasma membrane [H+]-ATPase of Neurospora crassa. I. Inhibition by N-ethylmaleimide and trypsin.
Brooker, R. J. & Slayman, C. W., Jul 25 1983, In: Journal of Biological Chemistry. 258, 14, p. 8827-8832 6 p.
Research output: Contribution to journal › Article › peer-review
22 Scopus citations
Effects of Mg2+ ions on the plasma membrane [H+]-ATPase of Neurospora crassa. II. Kinetic studies.
Brooker, R. J. & Slayman, C. W., Jul 25 1983, In: Journal of Biological Chemistry. 258, 14, p. 8833-8838 6 p.
Research output: Contribution to journal › Article › peer-review
32 Scopus citations
1982

Inhibition of the plasma membrane [H⁺]-ATPase of Neurospora crassa by N-ethylmaleimide. Protection by nucleotides
Brooker, R. J. & Slayman, C. W., 1982, In: Journal of Biological Chemistry. 257, 20, p. 12051-12055 5 p.
Research output: Contribution to journal › Article › peer-review

38 Scopus citations