1983 …2024
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Research Output 1983 2019

2001
6 Citations (Scopus)
Pyridoxamine
Fatty Acid-Binding Proteins
Transaminases
Fatty acids
Derivatives
34 Citations (Scopus)

Enzyme design by chemical modification of protein scaffolds

Tann, C. M., Qi, D. & Distefano, M. D., Dec 1 2001, In : Current Opinion in Chemical Biology. 5, 6, p. 696-704 9 p.

Research output: Contribution to journalReview article

Chemical modification
Scaffolds
Enzymes
Proteins
Genetic Techniques
28 Citations (Scopus)

Enzymes by design: Chemogenetic assembly of transamination active sites containing lysine residues for covalent catalysis

Häring, D. & Distefano, M. D., May 1 2001, In : Bioconjugate Chemistry. 12, 3, p. 385-390 6 p.

Research output: Contribution to journalArticle

Pyridoxamine
Transaminases
Catalysis
Lysine
Catalytic Domain
228 Citations (Scopus)

Generation of new enzymes via covalent modification of existing proteins

Qi, D., Tann, C. M., Haring, D. & Distefano, M. D., Oct 1 2001, In : Chemical Reviews. 101, 10, p. 3081-3111 31 p.

Research output: Contribution to journalArticle

Mutagenesis
Cysteine
Catalyst activity
Catalysts
Enzymes
3 Citations (Scopus)

Preparation and application of G protein γ subunit-derived peptides incorporating a photoactive isoprenoid

Kale, T. A., Turek, T. C., Chang, V., Gautam, N. & Distefano, M. D., Dec 1 2001, In : Methods in Enzymology. 344, p. 245-258 14 p.

Research output: Contribution to journalReview article

GTP-Binding Protein gamma Subunits
Terpenes
Peptides
11 Citations (Scopus)

Specific host-guest interactions in a protein-based artificial transaminase

Häring, D. & Distefano, M. D., Jan 1 2001, In : Bioorganic and Medicinal Chemistry. 9, 9, p. 2461-2466 6 p.

Research output: Contribution to journalArticle

Pyridoxamine
Transaminases
Substrates
Proteins
Derivatives
14 Citations (Scopus)

Stereochemical analysis of the reaction catalyzed by human protein geranylgeranyl transferase

Clausen, V. A., Edelstein, R. L. & Distefano, M. D., Apr 3 2001, In : Biochemistry. 40, 13, p. 3920-3930 11 p.

Research output: Contribution to journalArticle

Dipeptides
Transferases
Cathepsin A
Stereochemistry
Terpenes
51 Citations (Scopus)

Synthesis of farnesyl diphosphate analogues containing ether-linked photoactive benzophenones and their application in studies of protein prenyltransferases

Turek, T. C., Gaon, I., Distefano, M. D. & Strickland, C. L., May 18 2001, In : Journal of Organic Chemistry. 66, 10, p. 3253-3264 12 p.

Research output: Contribution to journalArticle

Benzophenones
Dimethylallyltranstransferase
Ether
Terpenes
Substrates
2000
9 Citations (Scopus)

Synthesis of a cationic pyridoxamine conjugation reagent and application to the mechanistic analysis of an artificial transaminase

Kuang, H., Häring, D., Qi, D., Mazhary, A. & Distefano, M. D., Jan 1 2000, In : Bioorganic and Medicinal Chemistry Letters. 10, 18, p. 2091-2095 5 p.

Research output: Contribution to journalArticle

Pyridoxamine
Transaminases
Pyridoxal
Fatty Acid-Binding Proteins
Deprotonation
1999
23 Citations (Scopus)

Artificial metalloenzymes based on protein cavities: Exploring the effect of altering the metal ligand attachment position by site directed mutagenesis

Davies, R. R., Kuang, H., Qi, D., Mazhary, A., Mayaan, E. & Distefano, M. D., Jan 1 1999, In : Bioorganic and Medicinal Chemistry Letters. 9, 1, p. 79-84 6 p.

Research output: Contribution to journalArticle

Mutagenesis
Site-Directed Mutagenesis
Metals
Ligands
Hydrolysis
1998
2 Citations (Scopus)
Anodic oxidation
Acetonitrile
Sulfur
Cations
Carbon
48 Citations (Scopus)

Catalytic enantioselective reductive amination in a host-guest system based on a protein cavity [2]

Kuang, H. & Distefano, M. D., Feb 11 1998, In : Journal of the American Chemical Society. 120, 5, p. 1072-1073 2 p.

Research output: Contribution to journalLetter

Amination
Proteins
15 Citations (Scopus)
Mutagenesis
Enantioselectivity
Site-Directed Mutagenesis
Transaminases
Metal ions
21 Citations (Scopus)

Measurement of the α-secondary kinetic isotope effect for a prenyltransferase by MALDI mass spectrometry [2]

Weller, V. A. & Distefano, M. D., Aug 12 1998, In : Journal of the American Chemical Society. 120, 31, p. 7975-7976 2 p.

Research output: Contribution to journalLetter

Dimethylallyltranstransferase
Isotopes
Mass spectrometry
Kinetics
23 Citations (Scopus)

Stereochemical Analysis of the Reaction Catalyzed by Yeast Protein Farnesyltransferase

Edelstein, R. L., Weller, V. A., Distefano, M. D. & Tung, J. S., Aug 7 1998, In : Journal of Organic Chemistry. 63, 16, p. 5298-5299 2 p.

Research output: Contribution to journalArticle

Fungal Proteins
p21(ras) farnesyl-protein transferase
24 Citations (Scopus)

Structural characterization of two synthetic catalysts based on adipocyte lipid-binding protein

Ory, J. J., Mazhary, A., Kuang, H., Davies, R. R., Distefano, M. D. & Banaszak, L. J., Apr 1 1998, In : Protein Engineering. 11, 4, p. 253-261 9 p.

Research output: Contribution to journalArticle

Fatty Acid-Binding Proteins
Lipids
Catalysts
Proteins
Crystal structure
21 Citations (Scopus)

The design of protein-based catalysts using semisynthetic methods

Distefano, M. D., Kuang, H., Qi, D. & Aram, M., Jan 1 1998, In : Current Opinion in Structural Biology. 8, 4, p. 459-465 7 p.

Research output: Contribution to journalArticle

Pyridoxamine
Pyridoxal
Substrate Specificity
Site-Directed Mutagenesis
Transaminases
1997
2 Citations (Scopus)

An artificial metalloenzyme based on a protein cavity that catalyzes the enantio-selective hydrolysis of amino acid esters

Davies, R. R., Brown, M. L. & Distefano, M. D., Dec 1 1997, In : Protein Engineering. 10, 3333, 1 p.

Research output: Contribution to journalArticle

Hydrolysis
Esters
Amino Acids
Proteins
3 Citations (Scopus)
Enantioselectivity
Catalysts
Enzymes
Proteins

Artificial transaminases: Tuning the selectivity and rate of pyridoxamine catalyzed reactions in a protein cavity modified by site directed mutagenesis

Distefano, M. D., Kuang, H., Mazharx, A., Ory, J. & Banaszak, L. J., Dec 1 1997, In : FASEB Journal. 11, 9

Research output: Contribution to journalArticle

Pyridoxamine
pyridoxamine
Mutagenesis
site-directed mutagenesis
transaminases
106 Citations (Scopus)
Fatty Acid-Binding Proteins
Amides
Lipids
Hydrolysis
Esters
80 Citations (Scopus)

Formation of microscale gradients of protein using heterobifunctional photolinkers

Hypolite, C. L., McLernon, T. L., Adams, D. N., Chapman, K. E., Herbert, C. B., Huang, C-C., Distefano, M. D. & Hu, W-S., Sep 1 1997, In : Bioconjugate Chemistry. 8, 5, p. 658-663 6 p.

Research output: Contribution to journalArticle

Glycols
Phycoerythrin
Proteins
Lasers
Extracellular Matrix Proteins

Mechanistic and structural studies on protein prenyl transferases

Waller, V. A., Turek, T. C., Gaon, I. & Distefano, M. D., Dec 1 1997, In : FASEB Journal. 11, 9

Research output: Contribution to journalArticle

Transferases
transferases
pyrophosphates
Catalytic Domain
Proteins
147 Citations (Scopus)

Micropatterning gradients and controlling surface densities of photoactivatable biomolecules on self-assembled monolayers of oligo(ethylene glycol) alkanethiolates

Herbert, C. B., McLernon, T. L., Hypolite, C. L., Adams, D. N., Pikus, L., Huang, C-C., Fields, G. B., Letourneau, P. C., Distefano, M. D. & Hu, W-S., Jan 1 1997, In : Chemistry and Biology. 4, 10, p. 731-737 7 p.

Research output: Contribution to journalArticle

Ethylene Glycol
Cell Shape
Biomolecules
Self assembled monolayers
Cell Adhesion
20 Citations (Scopus)
Pyridoxamine
Fatty Acid-Binding Proteins
Mutagenesis
Enantioselectivity
Substrate Specificity
28 Citations (Scopus)
ras Proteins
Fungal Proteins
Terpenes
Transferases
Labeling

Photoaffinity labeling using photoreactive isoprenoid analogs

Edelstein, R. L. & Distefano, M. D., Dec 1 1997, In : FASEB Journal. 11, 9

Research output: Contribution to journalArticle

photoaffinity labeling
isoprenoids
Terpenes
Labeling
Prenylation

Structural characterization of two artificial enzymes based on adipocyte lipid binding protein

Ory, J. J., Kuang, H., Davies, R., Mazhary, A., Distefano, M. D. & Banaszak, L. J., Dec 1 1997, In : FASEB Journal. 11, 9

Research output: Contribution to journalArticle

Fatty Acid-Binding Proteins
adipocytes
binding proteins
Enzymes
lipids
30 Citations (Scopus)

Synthesis and evaluation of benzophenone-based photoaffinity labeling analogs of prenyl pyrophosphates containing stable amide linkages

Turek, T. C., Gaon, I., Gamache, D. & Distefano, M. D., Aug 19 1997, In : Bioorganic and Medicinal Chemistry Letters. 7, 16, p. 2125-2130 6 p.

Research output: Contribution to journalArticle

Diphosphates
Transferases
Amides
Labeling
Fungal Proteins
7 Citations (Scopus)

Synthesis and rapid purification of 32P-labeled photoactive analogs of farnesyl pyrophosphate

Turek, T. C., Gaon, I. & Distefano, M. D., Feb 1 1997, In : Journal of Labelled Compounds and Radiopharmaceuticals. 39, 2, p. 139-146 8 p.

Research output: Contribution to journalArticle

Purification
Alcohols
Thin Layer Chromatography
Thin layer chromatography
Dimethylallyltranstransferase
1 Citation (Scopus)

Tools for the study of prenylated proteins: Photoactive benzophenone and diazotrifluoropropionate containing molecules

Turck, T. C., Edelstein, R. L., Gaon, I., Singh, S. K., Weller, V. & Distefano, M. D., Dec 1 1997, In : Protein Engineering. 10, 3333, 1 p.

Research output: Contribution to journalArticle

Molecules
Proteins
benzophenone
3 Citations (Scopus)

Two-dimensional surface gradients of photoactivable R-phycoerythrin

Hypolite, C. L., McLernon, T., Adams, D., Herbert, C., Huang, C-C., Hu, W-S. & Distefano, M. D., Dec 1 1997, In : Protein Engineering. 10, 3333, 1 p.

Research output: Contribution to journalArticle

1996
23 Citations (Scopus)
Fungal Proteins
Esters
Dimethylallyltranstransferase
Kinetics
Photolysis
61 Citations (Scopus)

Enantioselective reductive amination of α-keto acids to α-amino acids by a pyridoxamine cofactor in a protein cavity

Kuang, H., Brown, M. L., Davies, R. R., Young, E. C. & Distefano, M. D., Nov 6 1996, In : Journal of the American Chemical Society. 118, 44, p. 10702-10706 5 p.

Research output: Contribution to journalArticle

Pyridoxamine
Keto Acids
Amination
Fatty Acid-Binding Proteins
Lipids
30 Citations (Scopus)

Farnesyl and geranylgeranyl pyrophosphate analogs incorporating benzoylbenzyl ethers: Synthesis and inhibition of yeast protein farnesyltransferase

Gaon, I., Turek, T. C. & Distefano, M. D., Dec 2 1996, In : Tetrahedron Letters. 37, 49, p. 8833-8836 4 p.

Research output: Contribution to journalArticle

Fungal Proteins
Ethers
Benzophenones
Photolysis
Ether
47 Citations (Scopus)

Photoactive analogs of farnesyl pyrophosphate containing benzoylbenzoate esters: Synthesis and application to photoaffinity labeling of yeast protein farnesyltransferase

Gaon, I., Turek, T. C., Weller, V. A., Edelstein, R. L., Singh, S. K. & Distefano, M. D., Nov 1 1996, In : Journal of Organic Chemistry. 61, 22, p. 7738-7745 8 p.

Research output: Contribution to journalArticle

Fungal Proteins
Labeling
Esters
Photolysis
ras Proteins
1993
35 Citations (Scopus)

Cooperative Oligonucleotide-Directed Triple Helix Formation at Adjacent DNA Sites

Colocci, N., Distefano, M. D. & Dervan, P. B., May 1 1993, In : Journal of the American Chemical Society. 115, 11, p. 4468-4473 6 p.

Research output: Contribution to journalArticle

Spermine
Oligonucleotides
Oligodeoxyribonucleotides
Base Pairing
Free energy
18 Citations (Scopus)
Dimerization
Oligonucleotides
DNA
Bacteriophages
Nucleic Acids
1992
19 Citations (Scopus)

Ligand-Promoted Dimerization of Oligonucleotides Binding Cooperatively to DNA

Distefano, M. D. & Dervan, P. B., Dec 1 1992, In : Journal of the American Chemical Society. 114, 27, p. 11006-11007 2 p.

Research output: Contribution to journalLetter

Dimerization
Oligonucleotides
DNA
Ligands
1991
45 Citations (Scopus)

Cooperative Binding of Oligonucleotides to DNA by Triple Helix Formation: Dimerizatinn via Watson-Crick Hydrogen Bonds

Distefano, M. D., Shin, J. A. & Dervan, P. B., Jul 1 1991, In : Journal of the American Chemical Society. 113, 15, p. 5901-5902 2 p.

Research output: Contribution to journalArticle

Oligonucleotides
Hydrogen
Hydrogen bonds
DNA
165 Citations (Scopus)

Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Schiering, N., Kabsch, W., Moore, M. J., Distefano, M. D., Walsh, C. T. & Pai, E. F., Jan 1 1991, In : Nature. 352, 6331, p. 168-172 5 p.

Research output: Contribution to journalArticle

Bacillus
Flavin-Adenine Dinucleotide
Ions
Mercury
NADP
1990
59 Citations (Scopus)
Cysteine
Catalytic Domain
Oxidation-Reduction
Detoxification
Reducing Agents
18 Citations (Scopus)

An EXAFS Investigation of Hg(II) Binding to Mercuric Reductase: Comparative Analysis of the Wild-Type Enzyme and a Mutant Enzyme Generated by Site-Directed Mutagenesis

Raybuck, S. A., Distefano, M. D., Walsh, C. T., Teo, B. K. & Johnson, W. O., Jan 1 1990, In : Journal of the American Chemical Society. 112, 5, p. 1983-1989 7 p.

Research output: Contribution to journalArticle

Mutagenesis
Site-Directed Mutagenesis
Enzymes
Flavin-Adenine Dinucleotide
Cysteine
103 Citations (Scopus)

Organomercurial Lyase and Mercuric Ion Reductase: Nature’s Mercury Detoxification Catalysts

Moore, M. J., Distefano, M. D., Zydowsky, L. D., Cummings, R. T. & Walsh, C. T., Sep 1 1990, In : Accounts of Chemical Research. 23, 9, p. 301-308 8 p.

Research output: Contribution to journalArticle

Detoxification
Mercury
Oxidoreductases
Ions
Catalysts
95 Citations (Scopus)

Use of a Site-Directed Triple Mutant To Trap Intermediates: Demonstration That the Flavin C(4a)-Thiol Adduct and Reduced Flavin Are Kinetically Competent Intermediates in Mercuric Ion Reductase

Miller, S. M., Massey, V., Ballou, D., Williams, C. H., Distefano, M. D., Moore, M. J. & Walsh, C. T., Mar 1 1990, In : Biochemistry. 29, 11, p. 2831-2841 11 p.

Research output: Contribution to journalArticle

Sulfhydryl Compounds
Oxidoreductases
Demonstrations
Ions
Enzymes
1989
80 Citations (Scopus)

Evidence for the Participation of Cys 558 and Cys 559 at the Active Site of Mercuric Reductase

Miller, S. M., Moore, M. J., Massey, V., Williams, C. H., Distefano, M. D., Bailou, D. P. & Walsh, C. T., Jan 1 1989, In : Biochemistry. 28, 3, p. 1194-1205 12 p.

Research output: Contribution to journalArticle

Disulfides
Catalytic Domain
Sulfhydryl Compounds
Flavin-Adenine Dinucleotide
NADP
43 Citations (Scopus)

Mutagenesis of the Redox-Active Disulfide in Mercuric Ion Reductase: Catalysis by Mutant Enzymes Restricted to Flavin Redox Chemistry

Distefano, M. D., Au, K. G. & Walsh, C. T., Jan 1 1989, In : Biochemistry. 28, 3, p. 1168-1183 16 p.

Research output: Contribution to journalArticle

Mutagenesis
Catalysis
Disulfides
Oxidation-Reduction
Oxidoreductases
16 Citations (Scopus)

Purification, crystallization, and preliminary X-ray diffraction studies of the flavoenzyme mercuric ion reductase from Bacillus sp. strain RC607

Moore, M. J., Distefano, M. D., Walsh, C. T., Schiering, N. & Pai, E. F., Jan 1 1989, In : Journal of Biological Chemistry. 264, 24, p. 14386-14388 3 p.

Research output: Contribution to journalArticle

Bacilli
Crystallization
X-Ray Diffraction
Bacillus
Purification
1988
76 Citations (Scopus)

Molecular basis of bacterial resistance to organomercurial and inorganic mercuric salts.

Walsh, C. T., Distefano, M. D., Moore, M. J., Shewchuk, L. M. & Verdine, G. L., Feb 1 1988, In : The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2, 2, p. 124-130 7 p.

Research output: Contribution to journalReview article

organomercury compounds
structural genes
operon
mercury
transcription (genetics)
Flavoproteins
Mercury
Mutagenesis
Disulfides
Cysteine