If you made any changes in Pure, your changes will be visible here soon.

Research Output 2000 2019

Filter
Article
2019

Variable cellular decision-making behavior in a constant synthetic network topology

Shah, N. A. & Sarkar, C. A., May 14 2019, In : BMC bioinformatics. 20, 1, 237.

Research output: Contribution to journalArticle

Open Access
Gene Dosage
Network Topology
Decision Making
Decision making
Topology
2018
7 Citations (Scopus)

RNA Thermometers for the PURExpress System

Sadler, F. W., Dodevski, I. & Sarkar, C., Jan 19 2018, In : ACS Synthetic Biology. 7, 1, p. 292-296 5 p.

Research output: Contribution to journalArticle

Thermometers
RNA
Gene expression
Temperature
Synthetic Biology
2016
6 Citations (Scopus)

Toward bioremediation of methylmercury using silica encapsulated Escherichia coli harboring the mer operon

Kane, A. L., Al-Shayeb, B., Holec, P. V., Rajan, S., Le Mieux, N. E., Heinsch, S. C., Psarska, S., Aukema, K. G., Sarkar, C. A., Nater, E. A. & Gralnick, J. A., Jan 1 2016, In : PloS one. 11, 1, 0147036.

Research output: Contribution to journalArticle

methylmercury compounds
Environmental Biodegradation
Bioremediation
bioremediation
operon
2015

Computationally guided design of robust gene circuits

Shah, N. A. & Sarkar, C., Jan 1 2015, In : Methods in Molecular Biology. 1244, p. 167-178 12 p.

Research output: Contribution to journalArticle

Synthetic Biology
Gene Regulatory Networks
2 Citations (Scopus)

Robust hematopoietic progenitor cell commitment in the presence of a conflicting cue

Shah, N. A., Levesque, M. J., Raj, A. & Sarkar, C., Jan 1 2015, In : Journal of cell science. 128, 15, p. 3009-3017 9 p.

Research output: Contribution to journalArticle

Hematopoietic Stem Cells
Cues
Erythropoiesis
Megakaryocyte-Erythroid Progenitor Cells
Transcription Factors
2014
9 Citations (Scopus)

Alternatively spliced, truncated GCSF receptor promotes leukemogenic properties and sensitivity to JAK inhibition

Mehta, H. M., Futami, M., Glaubach, T., Lee, D. W., Andolina, J. R., Yang, Q., Whichard, Z., Quinn, M., Lu, H. F., Kao, W. M., Przychodzen, B., Sarkar, C., Minella, A., Maciejewski, J. P. & Corey, S. J., Jan 1 2014, In : Leukemia. 28, 5, p. 1041-1051 11 p.

Research output: Contribution to journalArticle

Granulocyte Colony-Stimulating Factor Receptors
Janus Kinases
Names
Protein Isoforms
Acute Myeloid Leukemia
1 Citation (Scopus)

NP-Sticky: A web server for optimizing DNA ligation with non-palindromic sticky ends

Ng, D. T. W. & Sarkar, C., Apr 17 2014, In : Journal of Molecular Biology. 426, 8, p. 1861-1869 9 p.

Research output: Contribution to journalArticle

Ligation
DNA
Thermodynamics
Molecular Biology
2013
41 Citations (Scopus)

Effective phagocytosis of low Her2 tumor cell lines with engineered, aglycosylated igg displaying high FcγRIIa affinity and selectivity

Jung, S. T., Kelton, W., Kang, T. H., Ng, D. T. W., Andersen, J. T., Sandlie, I., Sarkar, C. A. & Georgiou, G., Feb 15 2013, In : ACS Chemical Biology. 8, 2, p. 368-375 8 p.

Research output: Contribution to journalArticle

Cytophagocytosis
Tumor Cell Line
Phagocytosis
Tumors
Immunoglobulin G
38 Citations (Scopus)

Engineering signal peptides for enhanced protein secretion from Lactococcus lactis

Ng, D. T. W. & Sarkar, C. A., Jan 1 2013, In : Applied and environmental microbiology. 79, 1, p. 347-356 10 p.

Research output: Contribution to journalArticle

Lactococcus lactis
protein secretion
Protein Sorting Signals
signal peptide
secretion
7 Citations (Scopus)

Evidence for context-dependent complementarity of non-shine-dalgarno ribosome binding sites to Escherichia coli rRNA

Barendt, P. A., Shah, N. A., Barendt, G. A., Kothari, P. A. & Sarkar, C. A., May 17 2013, In : ACS Chemical Biology. 8, 5, p. 958-966 9 p.

Research output: Contribution to journalArticle

Ribosomal RNA
Ribosomes
Escherichia coli
Binding Sites
Messenger RNA
4 Citations (Scopus)

Streamlined protocol for mRNA display

Barendt, P. A., Ng, D. T. W., McQuade, C. N. & Sarkar, C., Feb 11 2013, In : ACS Combinatorial Science. 15, 2, p. 77-81 5 p.

Research output: Contribution to journalArticle

Ankyrin Repeat
Display devices
Messenger RNA
Purification
Peptides
2012
9 Citations (Scopus)

Broad-specificity mrna-rrna complementarity in efficient protein translation

Barendt, P. A., Shah, N. A., Barendt, G. A. & Sarkar, C., Mar 1 2012, In : PLoS genetics. 8, 3, e1002598.

Research output: Contribution to journalArticle

bacteriophage
complementarity
Protein Biosynthesis
eukaryote
ribosomes
6 Citations (Scopus)

Model-guided ligation strategy for optimal assembly of DNA libraries

Ng, D. T. W. & Sarkar, C. A., Oct 1 2012, In : Protein Engineering, Design and Selection. 25, 10, p. 669-678 10 p.

Research output: Contribution to journalArticle

Gene Library
Ligation
DNA
Display devices
Molecular biology
13 Citations (Scopus)

Transient Noise Amplification and Gene Expression Synchronization in a Bistable Mammalian Cell-Fate Switch

Palani, S. & Sarkar, C., Mar 29 2012, In : Cell Reports. 1, 3, p. 215-224 10 p.

Research output: Contribution to journalArticle

Gene expression
Amplification
Noise
Synchronization
Cells
2011
21 Citations (Scopus)

Nisin-inducible secretion of a biologically active single-chain insulin analog by Lactococcus lactis NZ9000

Ng, D. T. W. & Sarkar, C. A., Aug 1 2011, In : Biotechnology and bioengineering. 108, 8, p. 1987-1996 10 p.

Research output: Contribution to journalArticle

Nisin
Lactococcus lactis
Insulin
Cell growth
Small Intestine
61 Citations (Scopus)

Robust network topologies for generating switch-like cellular responses

Shah, N. A. & Sarkar, C. A., Jun 1 2011, In : PLoS Computational Biology. 7, 6, e1002085.

Research output: Contribution to journalArticle

Gene Regulatory Networks
Network Topology
topology
Switch
Switches
25 Citations (Scopus)

Synthetic conversion of a graded receptor signal into a tunable, reversible switch

Palani, S. & Sarkar, C. A., Apr 7 2011, In : Molecular Systems Biology. 7, 480.

Research output: Contribution to journalArticle

Receptor
Switch
Switches
Artificial Receptors
Synthetic Biology
112 Citations (Scopus)

Tunable signal processing in synthetic MAP kinase cascades

OShaughnessy, E. C., Palani, S., Collins, J. J. & Sarkar, C. A., Jan 7 2011, In : Cell. 144, 1, p. 119-131 13 p.

Research output: Contribution to journalArticle

MAP Kinase Signaling System
Signal processing
Phosphotransferases
Scaffolds
Chemical activation
2010
33 Citations (Scopus)

Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution

Zahnd, C., Sarkar, C. A. & Plückthun, A., Apr 1 2010, In : Protein Engineering, Design and Selection. 23, 4, p. 175-184 10 p.

Research output: Contribution to journalArticle

Libraries
Antigens
Experiments
Binders
Display devices
2009
19 Citations (Scopus)
Transcription factors
Minimal Model
Cues
Transcription Factor
Cell
29 Citations (Scopus)

Selection and characterization of DARPins specific for the neurotensin receptor 1

Milovnik, P., Ferrari, D., Sarkar, C. & Plückthun, A., Jun 1 2009, In : Protein Engineering, Design and Selection. 22, 6, p. 357-366 10 p.

Research output: Contribution to journalArticle

Ankyrin Repeat
G-Protein-Coupled Receptors
Proteins
Epitopes
Detergents
2008
124 Citations (Scopus)

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

Sarkar, C. A., Dodevski, I., Kenig, M., Dudli, S., Mohr, A., Hermans, E. & Plückthun, A., Sep 30 2008, In : Proceedings of the National Academy of Sciences of the United States of America. 105, 39, p. 14808-14813 6 p.

Research output: Contribution to journalArticle

Pichia
Amino Acid Substitution
G-Protein-Coupled Receptors
Eukaryota
Membrane Proteins
29 Citations (Scopus)

Positive receptor feedback during lineage commitment can generate ultrasensitivity to ligand and confer robustness to a bistable switch

Palani, S. & Sarkar, C., Aug 15 2008, In : Biophysical Journal. 95, 4, p. 1575-1589 15 p.

Research output: Contribution to journalArticle

Erythropoietin Receptors
Erythropoietin
Ligands
Transcription Factors
GATA Transcription Factors
2005
100 Citations (Scopus)

Protein PEGylation decreases observed target association rates via a dual blocking mechanism

Kubetzko, S., Sarkar, C. A. & Plückthun, A., Nov 1 2005, In : Molecular Pharmacology. 68, 5, p. 1439-1454 16 p.

Research output: Contribution to journalArticle

Immunoglobulin Fragments
Antigens
Single-Chain Antibodies
Proteins
Serum
2003
33 Citations (Scopus)

Cell-level pharmacokinetic model of granulocyte colony-stimulating factor: Implications for ligand lifetime and potency in vivo

Sarkar, C. A. & Lauffenburger, D. A., Jan 1 2003, In : Molecular Pharmacology. 63, 1, p. 147-158 12 p.

Research output: Contribution to journalArticle

Granulocyte Colony-Stimulating Factor
Pharmacokinetics
Granulocyte Colony-Stimulating Factor Receptors
Ligands
Neutrophils
27 Citations (Scopus)

Parsing the effects of binding, signaling, and trafficking on the mitogenic potencies of granulocyte colony-stimulating factor analogues

Sarkar, C. A., Lowenhaupt, K., Wang, P. J., Horan, T. & Lauffenburger, D. A., May 1 2003, In : Biotechnology Progress. 19, 3, p. 955-964 10 p.

Research output: Contribution to journalArticle

granulocyte colony-stimulating factor
Granulocyte Colony-Stimulating Factor
cytokines
Cytokine Receptors
Cytokines
28 Citations (Scopus)

ph dependence of structural stability of interleukin-2 and granulocyte colony-stimulating factor

Ricci, M. S., Sarkar, C. A., Fallon, E. M., Lauffenburger, D. A. & Brems, D. N., May 1 2003, In : Protein Science. 12, 5, p. 1030-1038 9 p.

Research output: Contribution to journalArticle

Granulocyte Colony-Stimulating Factor
Interleukin-2
Cytokines
Ligands
Denaturation
2002
108 Citations (Scopus)

Rational cytokine design for increased lifetime and enhanced potency using pH-activated “histidine switching”

Sarkar, C. A., Lowenhaupt, K., Horan, T., Boone, T. C., Tidor, B. & Lauffenburger, D. A., Jan 1 2002, In : Nature biotechnology. 20, 9, p. 908-913 6 p.

Research output: Contribution to journalArticle

Histidine
Half-Life
Recycling
Substitution reactions
Cytokines