Research Output 1971 2020

2020

Intrinsically disordered HAX-1 regulates Ca2+ cycling by interacting with lipid membranes and the phospholamban cytoplasmic region

Larsen, E. K., Weber, D. K., Wang, S., Gopinath, T., Blackwell, D. J., Dalton, M. P., Robia, S. L., Gao, J. & Veglia, G., Jan 1 2020, In : Biochimica et Biophysica Acta - Biomembranes. 1862, 1, 183034.

Research output: Contribution to journalArticle

Membrane Lipids
Reticulum
Calcium-Transporting ATPases
Cell Membrane
Cyclic AMP-Dependent Protein Kinases

Viral expression of a SERCA2a-activating PLB mutant improves calcium cycling and synchronicity in dilated cardiomyopathic hiPSC-CMs

Stroik, D. R., Ceholski, D. K., Bidwell, P. A., Mleczko, J., Thanel, P. F., Kamdar, F., Autry, J. M., Cornea, R. L. & Thomas, D. D., Jan 2020, In : Journal of Molecular and Cellular Cardiology. 138, p. 59-65 7 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Cardiac Myocytes
Fluorescence Resonance Energy Transfer
HEK293 Cells
Calcium
2019

An enhanced molecular tension sensor based on bioluminescence resonance energy transfer (BRET)

Aird, E. J., Tompkins, K. J. & Gordon, W. R., Apr 24 2019

Research output: Other contribution

1 Citation (Scopus)

A new regulatory mechanism for Raf kinase activation, retinoic acid-bound Crabp1

Wook Park, S., Nhieu, J., Persaud, S. D., Miller, M. C., Xia, Y., Lin, Y. W., Lin, Y. L., Kagechika, H., Mayo, K. H. & Wei, L. N., Dec 1 2019, In : Scientific reports. 9, 1, 10929.

Research output: Contribution to journalArticle

Open Access
Retinoic Acid Receptors
Fibrosarcoma
Tretinoin
Phosphotransferases
Intercellular Signaling Peptides and Proteins
1 Citation (Scopus)

An intramembrane sensory circuit monitors sortase A-mediated processing of streptococcal adhesins

Hall, J. W., Lima, B. P., Herbomel, G. G., Gopinath, T., McDonald, L. A., Shyne, M. T., Lee, J. K., Kreth, J., Ross, K. F., Veglia, G. & Herzberg, M. C., May 7 2019, In : Science signaling. 12, 580, eaas9941.

Research output: Contribution to journalArticle

Biofilms
Bacteria
Cell membranes
Networks (circuits)
Processing
2 Citations (Scopus)

A supramolecular synthon approach to design amorphous solid dispersions with exceptional physical stability

Duggirala, N. K., Li, J., Krishna Kumar, N. S., Tata, G. & Suryanarayanan, R., Jan 1 2019, In : Chemical Communications. 55, 39, p. 5551-5554 4 p.

Research output: Contribution to journalArticle

Dispersions
carbopol 940
X ray crystallography
Polymers
Pharmaceutical Preparations
Nuclear magnetic resonance spectroscopy
Membrane Proteins
Lipid bilayers
G-Protein-Coupled Receptors
Linewidth

Atomistic Models from Orientation and Distance Constraints Using EPR of a Bifunctional Spin Label

Binder, B. P., Thompson, A. R. & Thomas, D. D., Jul 23 2019, In : Biophysical journal. 117, 2, p. 319-330 12 p.

Research output: Contribution to journalArticle

Spin Labels
Myosins
Electron Spin Resonance Spectroscopy
Actins
Electrons

A toolkit for studying cell surface shedding of diverse transmembrane receptors

Hayward, A. N., Aird, E. J. & Gordon, W. R., Jun 2019, In : eLife. 8, e46983.

Research output: Contribution to journalArticle

Open Access
Proteolysis
Switches
Assays
Cell Surface Receptors
Cell Adhesion

Author Correction: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors (Nature Communications, (2019), 10, 1, (5222), 10.1038/s41467-019-13007-6)

Webber, B. R., Lonetree, C. L., Kluesner, M. G., Johnson, M. J., Pomeroy, E. J., Diers, M. D., Lahr, W. S., Draper, G. M., Slipek, N. J., Smeester, B. A., Lovendahl, K. N., McElroy, A. N., Gordon, W. R., Osborn, M. J. & Moriarity, B. S., Dec 1 2019, In : Nature communications. 10, 1, 5659.

Research output: Contribution to journalComment/debate

Open Access
Cell engineering
document markup languages
Cell Engineering
HTML
T-cells
2 Citations (Scopus)

Calmodulin inhibition of human RyR2 channels requires phosphorylation of RyR2-S2808 or RyR2-S2814

Walweel, K., Gomez-Hurtado, N., Rebbeck, R. T., Oo, Y. W., Beard, N. A., Molenaar, P., dos Remedios, C., van Helden, D. F., Cornea, R. L., Knollmann, B. C. & Laver, D. R., May 1 2019, In : Journal of Molecular and Cellular Cardiology. 130, p. 96-106 11 p.

Research output: Contribution to journalArticle

Ryanodine Receptor Calcium Release Channel
Calmodulin
Phosphorylation
Calcium-Calmodulin-Dependent Protein Kinases

Circulating tumor biomarkers in meningiomas reveal a signature of equilibrium between tumor growth and immune modulation

Erkan, E. P., Ströbel, T., Dorfer, C., Sonntagbauer, M., Weinhäusel, A., Saydam, N. & Saydam, O., Sep 1 2019, In : Frontiers in Oncology. 9, SEP, 1031.

Research output: Contribution to journalArticle

Open Access
Meningioma
Tumor Biomarkers
Growth
Neoplasms
Blood Proteins

Coding sequences of sarcoplasmic reticulum calcium ATPase regulatory peptides and expression of calcium regulatory genes in recurrent exertional rhabdomyolysis

Valberg, S. J., Soave, K., Williams, Z. J., Perumbakkam, S., Schott, M., Finno, C. J., Petersen, J. L., Fenger, C., Autry, J. M. & Thomas, D. D., Mar 1 2019, In : Journal of veterinary internal medicine. 33, 2, p. 933-941 9 p.

Research output: Contribution to journalArticle

Sarcoplasmic Reticulum Calcium-Transporting ATPases
rhabdomyolysis
Rhabdomyolysis
sarcoplasmic reticulum
Ca2-transporting ATPase
1 Citation (Scopus)

Converter domain mutations in myosin alter structural kinetics and motor function

Gunther, L. K., Rohde, J. A., Tang, W., Walton, S. D., Unrath, W. C., Trivedi, D. V., Muretta, J. M., Thomas, D. D. & Yengo, C. M., Jan 1 2019, In : Journal of Biological Chemistry. 294, 5, p. 1554-1567 14 p.

Research output: Contribution to journalArticle

Open Access
Myosins
Mutation
Kinetics
Adenosine Triphosphatases
Conformations

Corrigendum to “An improved conjugate vaccine technology; induction of antibody responses to the tumor vasculature” (Vaccine (2018) 36(21) (3054–3060), (S0264410X1830433X), (10.1016/j.vaccine.2018.03.064))

Huijbers, E. J. M., van Beijnum, J. R., Lê, C. T., Langman, S., Nowak-Sliwinska, P., Mayo, K. H. & Griffioen, A. W., Jul 9 2019, In : Vaccine. 37, 30, p. 4231-4232 2 p.

Research output: Contribution to journalComment/debate

Open Access
Conjugate Vaccines
Cancer Vaccines
Antibody Formation
vaccines
Technology

Crystal structure of the Wheat dwarf virus Rep domain Everett Blake A.

Everett, B. A., Litzau, L. A., Tompkins, K., Shi, K., Nelson, A., Aihara, H., Evans, R. L. E. & Gordon, W. R., Dec 1 2019, In : Acta Crystallographica Section F: Structural Biology Communications. 75, p. 744-749 6 p.

Research output: Contribution to journalArticle

wheat
Endonucleases
viruses
Viruses
Triticum

Cushing’s syndrome driver mutation disrupts protein kinase A allosteric network, altering both regulation and substrate specificity

Walker, C., Wang, Y., Olivieri, C., Karamafrooz, A., Casby, J., Bathon, K., Calebiro, D., Gao, J., Bernlohr, D. A., Taylor, S. S. & Veglia, G., Aug 28 2019, In : Science advances. 5, 8, eaaw9298.

Research output: Contribution to journalArticle

Open Access
mutations
proteins
disrupting
nucleotides
genes

Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy

Weber, D. K., Bader, T., Larsen, E. K., Wang, S., Gopinath, T., Distefano, M. & Veglia, G., 2019, Methods in Enzymology. Shukla, A. K. (ed.). Academic Press Inc., p. 281-304 24 p. (Methods in Enzymology; vol. 621).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nuclear magnetic resonance spectroscopy
Cysteine
Membrane Proteins
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Sarcomeres
Nucleic Acids
Troponin
Cytoplasm
Biological Phenomena
6 Citations (Scopus)

Discovery of Hydroxylase Activity for PqqB Provides a Missing Link in the Pyrroloquinoline Quinone Biosynthetic Pathway

Koehn, E. M., Latham, J. A., Armand, T., Evans, R. L., Tu, X., Wilmot, C. M., Iavarone, A. T. & Klinman, J. P., Mar 13 2019, In : Journal of the American Chemical Society. 141, 10, p. 4398-4405 8 p.

Research output: Contribution to journalArticle

PQQ Cofactor
Biosynthesis
Biosynthetic Pathways
Mixed Function Oxygenases
Iron

Dynamic allostery-based molecular workings of kinase:peptide complexes

Ahuja, L. G., Aoto, P. C., Kornev, A. P., Veglia, G. & Taylor, S. S., Jan 1 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 30, p. 15052-15061 10 p.

Research output: Contribution to journalArticle

Open Access
Phosphotransferases
Peptides
Cyclic AMP-Dependent Protein Kinases
Enzymes
Protein Kinases

Extracellular vesicles as carriers of suicide mRNA and/or protein in cancer therapy

Erkan, E. P., Saydam, N., Chen, C. C. & Saydam, O., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 87-96 10 p. (Methods in Molecular Biology; vol. 1895).

Research output: Chapter in Book/Report/Conference proceedingChapter

Suicide
Genetic Therapy
Messenger RNA
Prodrugs
Glioblastoma
2 Citations (Scopus)

Galectin-3 binds selectively to the terminal, non-reducing end of β(1→4)-galactans, with overall affinity increasing with chain length

Miller, M. C., Zheng, Y., Zhou, Y., Tai, G. & Mayo, K. H., Jan 1 2019, In : Glycobiology. 29, 1, p. 74-84 11 p.

Research output: Contribution to journalArticle

Galactans
Galectin 3
Chain length
Carbohydrates
Galectins
5 Citations (Scopus)

Globally correlated conformational entropy underlies positive and negative cooperativity in a kinase’s enzymatic cycle

Wang, Y., V.S, M., Kim, J. J., Li, G., Ahuja, L. G., Aoto, P., Taylor, S. S. & Veglia, G., Dec 1 2019, In : Nature communications. 10, 1, 799.

Research output: Contribution to journalArticle

Open Access
Entropy
enzymes
Phosphotransferases
entropy
cycles

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Webber, B. R., Lonetree, C. L., Kluesner, M. G., Johnson, M. J., Pomeroy, E. J., Diers, M. D., Lahr, W. S., Draper, G. M., Slipek, N. J., Smeester, B. S., Lovendahl, K. N., McElroy, A. N., Gordon, W. R., Osborn, M. J. & Moriarity, B. S., Dec 1 2019, In : Nature communications. 10, 1, 5222.

Research output: Contribution to journalArticle

Open Access
Cell engineering
Cell Engineering
T-cells
strands
nuclease
3 Citations (Scopus)
Magic angle spinning
Distance measurement
Membrane Proteins
Nuclear magnetic resonance
Biopolymers

Hybrid ligands with calixarene and thiodigalactoside groups: Galectin binding and cytotoxicity

Zhang, H., Ippel, H., Miller, M. C., Wong, T. J., Griffioen, A. W., Mayo, K. H. & Pieters, R. J., Aug 21 2019, In : Organic Chemistry Frontiers. 6, 16, p. 2981-2990 10 p.

Research output: Contribution to journalArticle

Calixarenes
Galectins
Cytotoxicity
Ligands
Galectin 1

Improving the quality of oriented membrane protein spectra using heat-compensated separated local field experiments

Wang, S., Gopinath, T. & Veglia, G., Nov 1 2019, In : Journal of Biomolecular Nmr. 73, 10-11, p. 617-624 8 p.

Research output: Contribution to journalArticle

Membrane Proteins
Hot Temperature
Experiments
Lipid bilayers
Chemical shift
1 Citation (Scopus)

Myosin lever arm orientation in muscle determined with high angular resolution using bifunctional spin labels

Savich, Y., Binder, B. P., Thompson, A. R. & Thomas, D. D., Aug 5 2019, In : The Journal of general physiology. 151, 8, p. 1007-1016 10 p.

Research output: Contribution to journalArticle

Open Access
Spin Labels
Myosins
Muscles
Myosin Light Chains
Cryoelectron Microscopy
1 Citation (Scopus)

NMR-based insight into galectin-3 binding to endothelial cell adhesion molecule CD146: Evidence for noncanonical interactions with the lectin's CRD β-sandwich F-face

Zhang, Z., Miller, M. C., Xu, X., Song, C., Zhang, F., Zheng, Y., Zhou, Y., Tai, G. & Mayo, K. H., Jul 19 2019, In : Glycobiology. 29, 8, p. 608-618 11 p.

Research output: Contribution to journalArticle

Galectin 3
Endothelial cells
Cell Adhesion Molecules
Lectins
Magnetic Resonance Spectroscopy
1 Citation (Scopus)

Noncompetitive inhibitors of TNFR1 probe conformational activation states

Lo, C. H., Schaaf, T. M., Grant, B. D., Lim, C. K. W., Bawaskar, P., Aldrich, C., Thomas, D. D. & Sachs, J. N., Jul 30 2019, In : Science signaling. 12, 592, eaav5637.

Research output: Contribution to journalArticle

Tumor Necrosis Factor Receptors
Chemical activation
Dimerization
Molecules
Ligands

Publisher Correction: A new regulatory mechanism for Raf kinase activation, retinoic acid-bound Crabp1 (Scientific Reports, (2019), 9, 1, (10929), 10.1038/s41598-019-47354-7)

Park, S. W., Nhieu, J., Persaud, S. D., Miller, M. C., Xia, Y., Lin, Y. W., Lin, Y. L., Kagechika, H., Mayo, K. H. & Wei, L. N., Dec 1 2019, In : Scientific reports. 9, 1, 17042.

Research output: Contribution to journalComment/debate

Open Access
raf Kinases
Tretinoin
1 Citation (Scopus)

Salicylate 5-Hydroxylase: Intermediates in Aromatic Hydroxylation by a Rieske Monooxygenase

Rogers, M. S. & Lipscomb, J. D., Jan 1 2019, In : Biochemistry.

Research output: Contribution to journalArticle

Hydroxylation
Mixed Function Oxygenases
Oxygenases
Gentisates
Electrons
5 Citations (Scopus)

Soluble methane monooxygenase

Banerjee, R., Jones, J. C. & Lipscomb, J. D., Jun 20 2019, In : Annual Review of Biochemistry. 88, p. 409-431 23 p.

Research output: Contribution to journalArticle

methane monooxygenase
Methane
Temperature
Enzymes
Chemical activation
1 Citation (Scopus)

Structure and Function of Tryptophan-Tyrosine Dyads in Biomimetic β Hairpins

McCaslin, T. G., Pagba, C. V., Chi, S. H., Hwang, H. J., Gumbart, J. C., Perry, J. W., Olivieri, C., Porcelli, F., Veglia, G., Guo, Z., McDaniel, M. & Barry, B. A., Apr 4 2019, In : Journal of Physical Chemistry B. 123, 13, p. 2780-2791 12 p.

Research output: Contribution to journalArticle

tyrosine
tryptophan
biomimetics
Biomimetics
Tryptophan

SUMO3 modification by PIAS1 modulates androgen receptor cellular distribution and stability

Yang, N., Liu, S., Qin, T., Liu, X., Watanabe, N., Mayo, K. H., Li, J. & Li, X., Nov 21 2019, In : Cell Communication and Signaling. 17, 1, 153.

Research output: Contribution to journalArticle

Open Access
Androgen Receptors
Sumoylation
Degradation
Lysine
Ubiquitin-Protein Ligases

Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor

Bader, T. K., Rappe, T. M., Veglia, G. & Distefano, M. D., Jan 1 2019, Methods in Enzymology. Wand, A. J. (ed.). Academic Press Inc., p. 207-238 32 p. (Methods in Enzymology; vol. 614).

Research output: Chapter in Book/Report/Conference proceedingChapter

Prenylation
Nuclear magnetic resonance
Peptides
Mating Factor
Yeast

T2* weighted Deconvolution of NMR Spectra: Application to 2D Homonuclear MAS Solid-State NMR of Membrane Proteins

Manu, V. S., Gopinath, T., Wang, S. & Veglia, G., Dec 1 2019, In : Scientific reports. 9, 1, 8225.

Research output: Contribution to journalArticle

Open Access
Membrane Proteins
Lipid Bilayers
Complex Mixtures
Artifacts
Magnetic Resonance Spectroscopy

Targeting the ensemble of heterogeneous tau oligomers in cells: A novel small molecule screening platform for tauopathies

Lo, C. H., Lim, C. K. W., Ding, Z., Wickramasinghe, S. P., Braun, A. R., Ashe, K. H., Rhoades, E., Thomas, D. D. & Sachs, J. N., Nov 2019, In : Alzheimer's and Dementia. 15, 11, p. 1489-1502 14 p.

Research output: Contribution to journalArticle

Tauopathies
Poisons
Biosensing Techniques
L 663536
Fluorescence Resonance Energy Transfer

The CaMKII inhibitor KN93-calmodulin interaction and implications for calmodulin tuning of NaV1.5 and RyR2 function

Johnson, C. N., Pattanayek, R., Potet, F., Rebbeck, R. T., Blackwell, D. J., Nikolaienko, R., Sequeira, V., Le Meur, R., Radwański, P. B., Davis, J. P., Zima, A. V., Cornea, R. L., Damo, S. M., Györke, S., George, A. L. & Knollmann, B. C., Sep 2019, In : Cell Calcium. 82, 102063.

Research output: Contribution to journalArticle

Ryanodine Receptor Calcium Release Channel
Calmodulin
Protein Kinase Inhibitors
Protein Kinases
Calcium-Calmodulin-Dependent Protein Kinase Type 2

Trajectory-Based Simulation of EPR Spectra: Models of Rotational Motion for Spin Labels on Proteins

Martin, P. D., Svensson, B., Thomas, D. D. & Stoll, S., Dec 5 2019, In : Journal of Physical Chemistry B. 123, 48, p. 10131-10141 11 p.

Research output: Contribution to journalArticle

Spin Labels
Paramagnetic resonance
Labels
electron paramagnetic resonance
Molecular dynamics
3 Citations (Scopus)

Unnatural verticilide enantiomer inhibits type 2 ryanodine receptor-mediated calcium leak and is antiarrhythmic

Batiste, S. M., Blackwell, D. J., Kim, K., Kryshtal, D. O., Gomez-Hurtado, N., Rebbeck, R. T., Cornea, R. L., Johnston, J. N. & Knollmann, B. C., Jan 1 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 11, p. 4810-4815 6 p.

Research output: Contribution to journalArticle

Open Access
Ryanodine Receptor Calcium Release Channel
Calcium
Biological Products
Cardiac Arrhythmias
Cardiac Myocytes
2 Citations (Scopus)

Use of paramagnetic systems to speed-up NMR data acquisition and for structural and dynamic studies

Kocman, V., Di Mauro, G. M., Veglia, G. & Ramamoorthy, A., Oct 1 2019, In : Solid State Nuclear Magnetic Resonance. 102, p. 36-46 11 p.

Research output: Contribution to journalReview article

data acquisition
Data acquisition
Nuclear magnetic resonance
Membranes
Relaxation time
1 Citation (Scopus)

Zooming in on protons: Neutron structure of protein kinase A trapped in a product complex

Gerlits, O., Weiss, K. L., Blakeley, M. P., Veglia, G., Taylor, S. S. & Kovalevsky, A., Jan 1 2019, In : Science Advances. 5, 3, eaav0482.

Research output: Contribution to journalArticle

Open Access
peptides
proteins
neutrons
adenosine diphosphate
protons
2018
4 Citations (Scopus)

Actin-myosin interaction: Structure, function and drug discovery

Guhathakurta, P., Prochniewicz, E. & Thomas, D. D., Sep 5 2018, In : International journal of molecular sciences. 19, 9, 2628.

Research output: Contribution to journalReview article

Open Access
myosins
Drug Discovery
Myosins
Actins
drugs
9 Citations (Scopus)

Adhesion/growth-regulatory galectins tested in combination: Evidence for formation of hybrids as heterodimers

Miller, M. C., Ludwig, A. K., Wichapong, K., Kaltner, H., Kopitz, J., Gabius, H. J. & Mayo, K. H., Mar 15 2018, In : Biochemical Journal. 475, 5, p. 1003-1018 16 p.

Research output: Contribution to journalArticle

Galectins
Adhesion
Galectin 1
Galectin 3
Growth
16 Citations (Scopus)

A dynamic mechanism for allosteric activation of aurora kinase a by activation loop phosphorylation

Ruff, E. F., Muretta, J. M., Thompson, A. R., Lake, E. W., Cyphers, S., Albanese, S. K., Hanson, S. M., Behr, J. M., Thomas, D. D., Chodera, J. D. & Levinson, N. M., Feb 21 2018, In : eLife. 7, e32766.

Research output: Contribution to journalArticle

Aurora Kinases
Phosphorylation
Chemical activation
Protein Kinases
Phosphotransferases
1 Citation (Scopus)

Age affects myosin relaxation states in skeletal muscle fibers of female but not male mice

Phung, L. A., Karvinen, S. M., Colson, B. A., Thomas, D. D. & Lowe, D. A., Sep 2018, In : PloS one. 13, 9, e0199062.

Research output: Contribution to journalArticle

Open Access
Skeletal Muscle Fibers
Myosins
myosin
Muscle
Fibers
3 Citations (Scopus)

ALARM NMR for HTS triage and chemical probe validation

Dahlin, J. L., Cuellar, M. E., Singh, G., Nelson, K., Strasser, J. M., Rappe, T., Xia, Y., Veglia, G. & Walters, M. A., Mar 1 2018, In : Current protocols in chemical biology. 10, 1, p. 91-117 27 p.

Research output: Contribution to journalArticle

Open Access
Triage
Magnetic Resonance Spectroscopy
Proteins
Antigens
Sulfhydryl Compounds