Olympus Fluoview FV1000 IX2 Inverted Confocal with FLIM Detector

    Equipment/facility: Equipment

      Asset Details

      Description

      This system is equipped with pulsed 405 and 485 nm lasers for FLIM. FLIM is the acronym for "Fluorescence Lifetime Imaging Microscopy" and is the technique that maps the spatial distribution of fluorescence lifetimes within microscopic images of fixed or living cells. The fluorescence lifetime measures the time that a population of fluorescent molecules spends in the excited state. The fluorescence lifetime does not change upon intensity variations and therefore lifetime measurements are not dependent on the local concentration of fluorophores, or variations in the optical path of the microscope, the local excitation light intensity, or on the local fluorescence detection efficiency.

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    • Research Output

      Physical-chemical interactions between dental materials surface, salivary pellicle and Streptococcus gordonii

      Sang, T., Ye, Z., Fischer, N. G., Skoe, E. P., Echeverría, C., Wu, J. & Aparicio, C., Jun 2020, In : Colloids and Surfaces B: Biointerfaces. 190, 110938.

      Research output: Contribution to journalArticle

    • Polyplexes Are Endocytosed by and Trafficked within Filopodia

      Ingle, N. P., Hexum, J. K. & Reineke, T. M., Apr 13 2020, In : Biomacromolecules. 21, 4, p. 1379-1392 14 p.

      Research output: Contribution to journalArticle

    • 1 Scopus citations

      Development of a Biolabeling System Using Ferromagnetic Nanowires

      Zhou, W., Um, J., Zhang, Y., Nelson, A. P., Nemati, Z., Modiano, J., Stadler, B. J. & Franklin, R. R., Jun 2019, In : IEEE Journal of Electromagnetics, RF and Microwaves in Medicine and Biology. 3, 2, p. 134-142 9 p., 8585028.

      Research output: Contribution to journalArticle

    • 3 Scopus citations