Nikon A1RHD Multiphoton Upright Confocal

    Equipment/facility: Equipment

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      Nikon A1RMP confocal is connected to an upright Nikon FN1 microscope featuring a Plan Apo LWD 25x water-immersion, NA 1.1 objective most suitable for imaging deep (it has a 2mm working distance) into a fixed or living sample. In addition to conventional and resonant visible laser confocal imaging, a Spectra Physics 15W Mai Tai eHP tunable IR laser and 4 non-descanned GaAsP detectors allow multiphoton visualization of multiple fluorophores located deep within the specimen. Visible lasers include 405, 457, 488, 514, 561 and 637 nm lines. The system has both galvonomic (4K) and resonance (720fps) scanners that can be used in concert simultaneously for photoactivation and MP imaging with enhanced hybrid descanned PMTs. An ASI piezo motorized stage(link is external) can be used for tiling or multiple-position imaging.


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