Sequence-Dependent Persistence Length of Long DNA

  • Han Cao (Creator)
  • Hui Min Chuang (Creator)
  • Kevin Dorfman (Creator)
  • Jeffrey G. Reifenberger (Creator)



Using a high-throughput genome-mapping approach, we obtained circa 50 million measurements of the

extension of internal human DNA segments in a 41 nm × 41 nm nanochannel. The underlying DNA

sequences, obtained by mapping to the reference human genome, are 2.5–393 kilobase pairs long and

contain percent GC contents between 32.5% and 60%. Using Odijk’s theory for a channel-confined

wormlike chain, these data reveal that the DNA persistence length increases by almost 20% as the percent

GC content increases. The increased persistence length is rationalized by a model, containing no adjustable

parameters, that treats the DNA as a statistical terpolymer with a sequence-dependent intrinsic persistence

length and a sequence-independent electrostatic persistence length.
Date made available2017
PublisherData Repository for the University of Minnesota

Cite this

Cao, H. (Creator), Chuang, H. (Creator), Dorfman, K. D. (Creator), Reifenberger, J. G. (Creator) (2017). Sequence-Dependent Persistence Length of Long DNA. Data Repository for the University of Minnesota. 10.13020/D6696N