Description
Using a high-throughput genome-mapping approach, we obtained circa 50 million measurements of the
extension of internal human DNA segments in a 41 nm × 41 nm nanochannel. The underlying DNA
sequences, obtained by mapping to the reference human genome, are 2.5–393 kilobase pairs long and
contain percent GC contents between 32.5% and 60%. Using Odijk’s theory for a channel-confined
wormlike chain, these data reveal that the DNA persistence length increases by almost 20% as the percent
GC content increases. The increased persistence length is rationalized by a model, containing no adjustable
parameters, that treats the DNA as a statistical terpolymer with a sequence-dependent intrinsic persistence
length and a sequence-independent electrostatic persistence length.
extension of internal human DNA segments in a 41 nm × 41 nm nanochannel. The underlying DNA
sequences, obtained by mapping to the reference human genome, are 2.5–393 kilobase pairs long and
contain percent GC contents between 32.5% and 60%. Using Odijk’s theory for a channel-confined
wormlike chain, these data reveal that the DNA persistence length increases by almost 20% as the percent
GC content increases. The increased persistence length is rationalized by a model, containing no adjustable
parameters, that treats the DNA as a statistical terpolymer with a sequence-dependent intrinsic persistence
length and a sequence-independent electrostatic persistence length.
| Date made available | 2017 |
|---|---|
| Publisher | Data Repository for the University of Minnesota |