Sequence-Dependent Persistence Length of Long DNA

  • Han Cao (Creator)
  • Hui-Min Chuang (Creator)
  • Kevin Dorfman (Creator)
  • Jeffrey G. Reifenberger (Creator)



Using a high-throughput genome-mapping approach, we obtained circa 50 million measurements of the

extension of internal human DNA segments in a 41 nm × 41 nm nanochannel. The underlying DNA

sequences, obtained by mapping to the reference human genome, are 2.5–393 kilobase pairs long and

contain percent GC contents between 32.5% and 60%. Using Odijk’s theory for a channel-confined

wormlike chain, these data reveal that the DNA persistence length increases by almost 20% as the percent

GC content increases. The increased persistence length is rationalized by a model, containing no adjustable

parameters, that treats the DNA as a statistical terpolymer with a sequence-dependent intrinsic persistence

length and a sequence-independent electrostatic persistence length.
Date made available2017
PublisherData Repository for the University of Minnesota

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