Raw data of: Settling selection of Chlamydomonas reinhardtii for samarium uptake.

Dataset

Description

Data are the raw results of the investigation entitled, Settling selection of Chlamydomonas reinhardtii for samarium uptake. This study uses an experimentally evolved C. reinhardtii (Martínez-Alesón García et al., 2021), selected under Samarium and acid pH conditions. We carry out further selection procedures to increase Sm sequestration. Control, ancestral, and selected populations of C. reinhardtii were characterized using a Coulter Counter multisizer 4, FlowCam 3.0, using Transmission electron microscopy (TEM), ray spectroscopy (Energy Dispersive X-ray (EDX)) and with an Imaging PAM fluorimeter. The populations studies were a C. reinhardtii wild-type control (control strain), Sm-uptake candidate strain (CSm0 strain) resulting from mixing thirteen Sm-adapted strains (refer to Martínez-Alesón García et al., 2021), and newly established strains label for the selection cycle as CSm1, CSm2, CSm3, and CSm4 strains. Files: Coulter Counter particles distributions of all strains: Coulter Counter raw data.zip Data from Control, CSm0, CSm1, CSm2, CSm3, and CSm4 strains grown in BG11 medium under laboratory conditions for 15 days. Microscopic images: Flow Cam raw images and files.zip FlowCam 3.0 Microphotographs and files were generated for all strains grown in BG11 medium under laboratory conditions for 15 days. Optic Microscopy microphotographs.zip Optic Microscopy Microphotographs measure morphological traits with ImageJ in CSm0 and CSm4 strains. TEM Images.zip Transmission electron microscopy (TEM) pictures were obtained with an electron microscope, JEOL-JEM 1400 (Jeol Ltd., Tokyo, Japan) from control and CSm4 strains. Data files generated from microscopy images: ImageJ analyses Raw data.xlsx: ImageJ analyses files. TEM-EDX analyses.zip Ray spectroscopy (Energy Dispersive X-ray (EDX)) spectrums obtained from TEM cuts. PAM raw data.zip PAM fluorescence Raw data of
Date made availableSep 29 2023
PublisherZENODO

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