Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis

The data deposited here are raw files, R code and mass spec, associated with the results presented in the paper, "Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis." These data have been made publicly available in keeping with the journal's data availability policy.

Description
R Code: Supplementary Data 1. Code for running kinetics simulations File name: Supplementary Data 1.R Mass Spectrometry .raw files: Supplementary Figure 4a-c. Mass spectrometric analysis of split borosin coexpressions. File name: 20200110_FSM1167_SonA+SonM_24.5min.raw Supplementary Figure 4d-j. Mass spectrometric analysis of split borosin coexpressions. File name: 20200909_KC1063_StrA+StrM_15min_redalk_18pm4.raw Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. File names: 20200220_FSM1178_kineticmodeling_t=2.raw 20200220_FSM1179_kineticmodeling_t=2.raw 20200220_FSM1180_kineticmodeling_t=8.raw 20200220_FSM1181_kineticmodeling_t=8.raw 20200220_FSM1182_kineticmodeling_t=20.raw 20200220_FSM1183_kineticmodeling_t=20.raw 20200220_FSM1184_kineticmodeling_t=40.raw 20200220_FSM1185_kineticmodeling_t=40.raw 20200220_FSM1186_kineticmodeling_t=60.raw 20200220_FSM1187_kineticmodeling_t=60.raw Supplementary Figure 25a. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190701_kc1007_His-SonA_Y93F.raw Supplementary Figure 25b. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190515_fsm1155_his-SonA-sonMT-R67K.raw Supplementary Figure 25c. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190515_fsm1154_his-sonA_sonMTR67A.raw Supplementary Figure 25d. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190701_kc1008_His-SonA_Y58F.raw Supplementary Figure 25e. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190515_fsm1156_his-sonA-SonMT-Y71F.raw Supplementary Figure 25f. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190701_kc1009_His-SonA_DBLMUT.raw

Funding information
Sponsorship: National Institutes of Health (R35 GM133475 to M.F.F.) and the University of Minnesota along with the BioTechnology Institute (M.F.F., M.H.E., W.A.H.)